The regulatory role of MiR-203 in oxidative stress induced cell injury through the CBS/H2S pathway
文献类型：期刊论文
| 作者 | Zhang, Qiuyan1,2,6; Shen, Zhuqing2,3; Shen, Yaqi2; Ma, Muye2; Jue, Hao2; Zhu, Yizhun2,4,5; Guo, Wei2 |
| 刊名 | NITRIC OXIDE-BIOLOGY AND CHEMISTRY
 |
| 出版日期 | 2022 |
| 卷号 | 118页码:31-38 |
| ISSN号 | 1089-8603 |
| 关键词 | Cerebral infarction Cystathionine-beta-synthase miRNA-203 Hydrogen sulfide |
| DOI | 10.1016/j.niox.2021.10.007 |
| 通讯作者 | Guo, Wei(guowei@fudan.edu.cn) |
| 英文摘要 | Hydrogen Sulfide (H2S) mediates biological effects in a variety of ways. Due to its strong reducing potential, H2S has been recognized to have an important role in oxidative stress induced hypoxia. It has been reported that H2S production and miRNA can mutually regulate each other. H2S is produced by the catalytic activity of cystathionine-beta-synthase (CBS), which is under the regulation of miRNAs. In this study, we used target gene prediction software, and identified miR-203 as a potential regulator of CBS. We verified this finding using an oxygen and glucose deprivation (OGD) hypoxia cell model in SH-SY5Y cells and pMIR-REPORTTM luciferase miRNA expression reporter vector. Furthermore, transfecting SH-SY5Y cells with miRNA agomir (agonist) and antagomir (antagonist) by lipofectamin RNAiMAX, we further validated miR-203 as a direct regulator of CBS. We also found that miR-203 protects from cell injury by regulating lipid peroxidation, cell apoptosis, and mitochondrial membrane potential. These findings suggest that while over-expression of miR-203 can aggravate OGD induced cell injury, inhibition of miR-203 can protect against OGD induced cell injury. Based on our data and that of others, we propose that miR-203 may regulate oxidative stress induced cell injury by regulating CBS expression and adjusting the levels of H2S production. |
| WOS关键词 | CYSTATHIONINE-BETA-SYNTHASE ; HYDROGEN-SULFIDE ; NEUROMODULATOR ; MICRORNA-21 |
| 资助项目 | National Natural Science Foundation of China[81872864] ; National Natural Science Foundation of China[81402919] ; FDUROP, Fudan's Undergraduate Research Opportunities Program[19079] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000721359900001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/298973]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, Wei |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Mat Med, Shanghai Inst Mat Med, Yantai Branch, Shanghai, Peoples R China 2.Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai Key Lab Bioact Small Mol, 826 Zhangheng Rd, Shanghai 201203, Peoples R China 3.Fudan Univ, Dept Pharm, Eye Ear Nose Throat Hosp, 83 Fenyang Rd, Shanghai 200031, Peoples R China 4.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China 5.Macau Univ Sci & Technol, Sch Pharm, Macau, Peoples R China 6.Yantai Univ, Key Lab Mol Pharmacol & Drug Evaluat, Minist Educ, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qiuyan,Shen, Zhuqing,Shen, Yaqi,et al. The regulatory role of MiR-203 in oxidative stress induced cell injury through the CBS/H2S pathway[J]. NITRIC OXIDE-BIOLOGY AND CHEMISTRY,2022,118:31-38. |
| APA | Zhang, Qiuyan.,Shen, Zhuqing.,Shen, Yaqi.,Ma, Muye.,Jue, Hao.,...&Guo, Wei.(2022).The regulatory role of MiR-203 in oxidative stress induced cell injury through the CBS/H2S pathway.NITRIC OXIDE-BIOLOGY AND CHEMISTRY,118,31-38. |
| MLA | Zhang, Qiuyan,et al."The regulatory role of MiR-203 in oxidative stress induced cell injury through the CBS/H2S pathway".NITRIC OXIDE-BIOLOGY AND CHEMISTRY 118(2022):31-38. |
入库方式： OAI收割
来源：上海药物研究所
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