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A simultaneous identification and quantification strategy for determination of sulfhydryl-containing metabolites in normal- and high-fat diet hamsters using stable isotope labeling combined with LC-MS

文献类型：期刊论文

作者Chang, Lu1,2; Lin, Feifei2,3; Cheng, Kai4,5,6; Li, Jiaomeng2; Sun, Xiaochu2; Figeys, Daniel4,5,6; Jiang, Jianlan1; Ye, Yang2,3; Liu, Jia2,7
刊名ANALYTICA CHIMICA ACTA
出版日期2021-11-01
卷号1184页码:13
关键词Sulfhydryl-containing metabolites Stable isotope labeling Identification Quantification Hyperlipidemia Oxidative stress
ISSN号0003-2670
DOI10.1016/j.aca.2021.339016
通讯作者Jiang, Jianlan(jljiang@tju.edu.cn) ; Ye, Yang(yye@simm.ac.cn) ; Liu, Jia(jia.liu@simm.ac.cn)
英文摘要Sulfur-containing metabolites are related to several physiologic disorders and metabolic diseases. In this study, a simultaneous identification and quantification strategy in one batch for determination of sulfhydryl-containing metabolites was developed using stable isotope labeling combined with liquid chromatography-tandem mass spectrometry (SIL-LC-MS). In the proposed method, a pair of isotope labeling reagents, D-0/D-5-N-ethylmaleimide (D-0/D-5-NEM), was used to derivatize sulfhydryl-containing metabolites in blood and plasma of normal-and high-fat-diet (NFD and HFD) hamsters for reduced (-SH) and total (-SH,-S-S-, S-glutathionylated proteins) analysis. Quality control (QC) samples and test samples were prepared for LC-MS analysis. First, both QC samples and stable isotope labeled internal standards were used to monitor the status of the instrument and ensure the reliability of the analysis. Subsequently, an inhouse database containing 45 sulfhydryl-containing metabolites was established by MS1 based on QC samples. Then, qualitatively differential sulfhydryl-containing metabolites were found by MS2 between the NFD and HFD hamsters of the test samples, including 3 in reduced and 8 in total analysis of blood samples, and 2 in reduced and 2 in total analysis of plasma samples. Next, in quantitative analysis, satisfied linearities for 6 sulfhydryl-containing metabolites were obtained with the correlation coefficient (R-2) > 0.99 and absolute quantification was carried out. The results showed that glutathione and cysteine have different concentrations in blood and plasma of hamsters. Finally, the correlation of sulfhydryl-containing metabolites with blood lipid and oxidative stress levels was determined, which provided insight into the hyperlipidemia-related oxidative stress. Taken together, the developed method of simultaneous identification with the inhouse database and MS2 and quantification with standards in one batch provides a promising strategy for the analysis of sulfhydryl-containing metabolites in biological samples, which may promote the in-depth investigation on sulfhydrylcontaining metabolites and related diseases. (C) 2021 Elsevier B.V. All rights reserved.
WOS关键词MASS-SPECTROMETRY ; HUMAN PLASMA ; THIOLS ; DERIVATIZATION ; HOMOCYSTEINE ; GLUTATHIONE ; SAMPLES
资助项目National Natural Science Foundation of China[21920102003] ; Science and Technology Commission of Shanghai Municipality[20430780300] ; Key-Area Research and Development Program of Guangdong Province[2020B0303070002]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000729823200010
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/299196]  
专题中国科学院上海药物研究所
通讯作者Jiang, Jianlan; Ye, Yang; Liu, Jia
作者单位1.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin 300350, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
4.Univ Ottawa, Fac Med, SIMM Univ Ottawa Joint Res Ctr Syst & Personalize, 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada
5.Univ Ottawa, Fac Med, Ottawa Inst Syst Biol, 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada
6.Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada
7.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310058, Peoples R China
推荐引用方式
GB/T 7714		 Chang, Lu,Lin, Feifei,Cheng, Kai,et al. A simultaneous identification and quantification strategy for determination of sulfhydryl-containing metabolites in normal- and high-fat diet hamsters using stable isotope labeling combined with LC-MS[J]. ANALYTICA CHIMICA ACTA,2021,1184:13.
APA Chang, Lu.,Lin, Feifei.,Cheng, Kai.,Li, Jiaomeng.,Sun, Xiaochu.,...&Liu, Jia.(2021).A simultaneous identification and quantification strategy for determination of sulfhydryl-containing metabolites in normal- and high-fat diet hamsters using stable isotope labeling combined with LC-MS.ANALYTICA CHIMICA ACTA,1184,13.
MLA Chang, Lu,et al."A simultaneous identification and quantification strategy for determination of sulfhydryl-containing metabolites in normal- and high-fat diet hamsters using stable isotope labeling combined with LC-MS".ANALYTICA CHIMICA ACTA 1184(2021):13.

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来源：上海药物研究所

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