Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy
文献类型：期刊论文
| 作者 | Du, Wei1,2,3; Pan, Dayi1; Xiang, Pan1; Xiong, Chaoya4; Zhang, Mingzhu4; Zhang, Qiong4; Tian, Yupeng4; Zhang, Zhongping4,5 ; Chen, Bo7; Luo, Kui1,6
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| 刊名 | ACS SENSORS
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| 出版日期 | 2021-11-26 |
| 卷号 | 6 |
| 关键词 | Zn complexes azide units histone probe STED nanoscopy super-resolution imaging |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.1c01287 |
| 通讯作者 | Chen, Bo(bo.chen@tongji.cdu.cn) ; Tian, Xiaohe(xiaohe.t@wchscu.cn) |
| 英文摘要 | Histones are the alkali proteins in eukaryotic somatic chromatin cells which constitute the nucleosome structure together with DNA. Their abnormality is often associated with multiple tumorigenesis and other human diseases. Nevertheless, a simple and efficient super-resolution method to visualize histone distribution at the subcellular level is still unavailable. Herein, a Zn(II) terpyridine complex with rich-electronic azide units, namely, TpZnA-His, was designed and synthesized. The initial in vitro and in silico studies suggested that this complex is able to detect histones rapidly and selectively via charge-charge interactions with the histone H3 subunit. Its live cell nuclear localization, red-emission tail, and large Stokes shift allowed super-resolution evaluation of histone distributions with a clear distinction against nuclear DNA. We were able to quantitatively conclude three histone morphology alternations in live cells including condensation, aggregation, and cavity during activating histone acetylation. This work offers a better understanding as well as a versatile tool to study histone-involved gene transcription, signal transduction, and differentiation in cells. |
| WOS关键词 | LABEL-FREE ; FLUORESCENCE |
| 资助项目 | National Natural Science Foundation of China[81621003] ; National Natural Science Foundation of China[321710012221871003] ; Postdoctoral Research Funding Schemes of Jiangsu Province (2021) |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000755689100012 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China ; Postdoctoral Research Funding Schemes of Jiangsu Province (2021) |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/127503]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Chen, Bo; Tian, Xiaohe |
| 作者单位 | 1.Sichuan Univ, West China Hosp, Huaxi Magnet Resonance Res Ctr HMRRC, Dept Radiol,Funct & Mol Imaging Key Lab Sichuan P, Chengdu 610041, Peoples R China 2.Nanjing Tech Univ NanjingTech, Key Lab Flexible Elect KLOFE, Nanjing 211816, Peoples R China 3.Nanjing Tech Univ NanjingTech, Inst Adv Mat IAM, Nanjing 211816, Peoples R China 4.Anhui Univ, Sch Life Sci, Inst Phys Sci & Informat Technol, Dept Chem, Hefei 230000, Peoples R China 5.Chinese Acad Sci, CAS Ctr Excellence Nanosci, Inst Intelligent Machines, Hefei 230031, Peoples R China 6.Chinese Acad Med Sci, Res Unit Psychoradiol, Chengdu 610041, Peoples R China 7.Tongji Univ, Sch Mat Sci & Engn, Shanghai 201804, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Wei,Pan, Dayi,Xiang, Pan,et al. Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy[J]. ACS SENSORS,2021,6. |
| APA | Du, Wei.,Pan, Dayi.,Xiang, Pan.,Xiong, Chaoya.,Zhang, Mingzhu.,...&Tian, Xiaohe.(2021).Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy.ACS SENSORS,6. |
| MLA | Du, Wei,et al."Terpyridine Zn(II) Complexes with Azide Units for Visualization of Histone Deacetylation in Living Cells under STED Nanoscopy".ACS SENSORS 6(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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