Aptamer-Functionalized Nanochannels for One-Step Detection of SARS-CoV-2 in Samples from COVID-19 Patients
文献类型：期刊论文
| 作者 | Shi, Liu1; Wang, Lin1; Ma, Xuemei1; Fang, Xiaona2,3; Xiang, Liangliang4; Yi, Yongxiang4; Li, Jinlong4; Luo, Zhaofeng2,3; Li, Genxi1,5 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2021-12-14 |
| 卷号 | 93 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.1c04156 |
| 通讯作者 | Li, Jinlong(jinlonglilab@njucm.edu.cn) ; Luo, Zhaofeng(lzf@ustc.edu.cn) ; Li, Genxi(genxili@nju.edu.cn) |
| 英文摘要 | With the outbreak of COVID-19, which is fast transmitting and highly contagious, the development of rapid, highly specific, and sensitive detection kits has become a research hotspot. The existing assay methods for SARS-CoV-2 are mainly based on enzymatic reactions, which require expensive reagents, hindering popular use, especially in resource-constrained areas. Herein, we propose an aptamer-based method for the assay of SARS-CoV-2 via binding of the spike protein using functionalized biomimetic nanochannels. To get the analogous effect of human ACE2, a receptor for the spike protein, the aptamer to bind to the spike Si protein has been first screened by a SELEX technique and then immobilized on the previously prepared nanochannels. In the presence of SARS-CoV-2, the changes in steric hindrance and charge density on the surface of the nanochannels will affect the ion transport, along with a rapid electrochemical response. Our method has been successfully applied to detect the viral particles in clinical pharyngeal swab specimens in one step without sample treatment. We expect this rapid, reagent-free, and sensitive assay method to be developed as a useful tool for diagnosing COVID-19. |
| 资助项目 | National Natural Science Foundation of China[81772593] ; Fundamental Research Funds for the Central Universities[14380131] ; Fundamental Research Funds for the Central Universities[YD2070002013] ; Fundamental Research Funds for the Central Universities[YD2070002016] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000753032200043 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/127624]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Li, Jinlong; Luo, Zhaofeng; Li, Genxi |
| 作者单位 | 1.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Aptamer Select Ctr, Inst Basic Med & Canc IBMC,Canc Hosp, Hangzhou 310022, Peoples R China 4.Nanjing Univ Chinese Med, Second Hosp Nanjing, Nanjing 210003, Peoples R China 5.Shanghai Univ, Ctr Mol Recognit & Biosensing, Sch Life Sci, Shanghai 200444, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Liu,Wang, Lin,Ma, Xuemei,et al. Aptamer-Functionalized Nanochannels for One-Step Detection of SARS-CoV-2 in Samples from COVID-19 Patients[J]. ANALYTICAL CHEMISTRY,2021,93. |
| APA | Shi, Liu.,Wang, Lin.,Ma, Xuemei.,Fang, Xiaona.,Xiang, Liangliang.,...&Li, Genxi.(2021).Aptamer-Functionalized Nanochannels for One-Step Detection of SARS-CoV-2 in Samples from COVID-19 Patients.ANALYTICAL CHEMISTRY,93. |
| MLA | Shi, Liu,et al."Aptamer-Functionalized Nanochannels for One-Step Detection of SARS-CoV-2 in Samples from COVID-19 Patients".ANALYTICAL CHEMISTRY 93(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。