Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
文献类型：期刊论文
| 作者 | Chen, Rui1; Zhang, Ningxia2; Zhou, Yubin3; Jing, Ji4 |
| 刊名 | FRONTIERS IN CELLULAR NEUROSCIENCE
 |
| 出版日期 | 2022-02-16 |
| 卷号 | 16 |
| 关键词 | proximity labeling TurboID optogenetics OptoID biotinylation |
| DOI | 10.3389/fncel.2022.801644 |
| 通讯作者 | Zhou, Yubin(yubinzhou@tamu.edu) ; Jing, Ji(jingji@ibmc.ac.cn) |
| 英文摘要 | Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells. The engineered biotin ligase TurboID is among the most widely adopted given its enhanced biotinylation efficiency, but it faces the background biotinylation complication that might confound proteomic data interpretation. To address this issue, we report herein a set of split TurboID variants that can be reversibly assembled by using light (designated "OptoID"), which enable optogenetic control of biotinylation based proximity labeling in living cells. OptoID could be further coupled with an engineered monomeric streptavidin that permits real-time monitoring of biotinylation with high temporal precision. These optical actuators and sensors will likely find broad applications in precise proximity proteomics and rapid detection of biotinylation in living cells. |
| WOS关键词 | STREPTAVIDIN MONOMER ; MEMBRANE RECRUITMENT ; BIOTIN REPRESSOR ; DIMERIZATION ; PROTEINS ; BINDING ; GROWTH ; LIGHT ; DIMER ; TOOL |
| 资助项目 | Welch Foundation[BE-1913-20190330] ; Cancer Hospital of the University of Chinese Academy of Sciences ; Institute of Basic Medicine and Cancer |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000764307900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Welch Foundation ; Cancer Hospital of the University of Chinese Academy of Sciences ; Institute of Basic Medicine and Cancer |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/127855]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhou, Yubin; Jing, Ji |
| 作者单位 | 1.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Oral & Maxillofacial Surg, Guangzhou, Peoples R China 2.Zhejiang Univ, Sir Run Run Shaw Hosp, Coll Med, Inst Clin Sci,Lab Canc Biol,Dept Med Oncol, Hangzhou, Peoples R China 3.Texas A&M Univ, Coll Med, Ctr Translat Canc Res Inst Biosci & Technol, Dept Translat Med Sci, Houston, TX 77843 USA 4.Univ Chinese Acad Sci, Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp,Inst Basic Med & Canc, Hangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Rui,Zhang, Ningxia,Zhou, Yubin,et al. Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells[J]. FRONTIERS IN CELLULAR NEUROSCIENCE,2022,16. |
| APA | Chen, Rui,Zhang, Ningxia,Zhou, Yubin,&Jing, Ji.(2022).Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells.FRONTIERS IN CELLULAR NEUROSCIENCE,16. |
| MLA | Chen, Rui,et al."Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells".FRONTIERS IN CELLULAR NEUROSCIENCE 16(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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