Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae
文献类型：期刊论文
| 作者 | Frericks, Jonathan; Munkacsi, Andrew1; Ritchie, Peter1; Luo, Yi-Bo; Lehnebach, Carlos A. |
| 刊名 | NEW ZEALAND JOURNAL OF BOTANY
 |
| 出版日期 | 2018 |
| 卷号 | 56期号:1页码:91-108 |
| ISSN号 | 0028-825X |
| 关键词 | Conservation germination mycorrhiza New Zealand orchid phylogeny taxonomy Tulasnella Spiranthes |
| DOI | 10.1080/0028825X.2017.1418398 |
| 文献子类 | Article |
| 英文摘要 | The New Zealand ladies' tresses Spiranthes novae-zelandiae is a terrestrial orchid currently classified as Threatened - Nationally Vulnerable. Although considered endemic to New Zealand, morphological similarity with the widespread Eurasian Spiranthes sinensis has cast doubt on its taxonomic and biogeographic status. Habitat destruction is the main threat to the survival of S.novae-zelandiae. Unfortunately, lack of information regarding its symbiotic fungal associates and technical expertise within New Zealand with symbiotic seed germination techniques have hindered its propagation from seed. In this study we examined the phylogenetic affinities of S.novae-zelandiae using nuclear (internal transcribed spacer) and chloroplast (trnL, trnS-G and matK) DNA sequences. We also explored the diversity of fungal symbionts associated with S.novae-zelandiae and identified the fungal symbiont that promotes seed germination and seedling development using DNA sequences and in vitro seed germination experiments. Bayesian Inference analyses showed that S.novae-zelandiae is nested within S.sinensis along with Spiranthes australis and Spiranthes aff. novae-zelandiae (CHR 518297; Motutangi), a morphological variant of uncertain taxonomic status from northern New Zealand. These results support earlier suggestions that a broader concept of S.sinensis is needed to include S.novae-zelandiae and many other taxa segregated from S.sinensis. Nine fungal Operational Taxonomic Units were isolated from the roots of S.novae-zelandiae but only one promoted seed germination and seedling development. DNA sequence analyses confirmed that this isolate was a strain of Tulasnella (anamorph: Epulorhiza); a widespread mycorrhizal fungus previously found in S.australis and S.sinensis. Lastly, we describe the germination process and the steps we followed to obtain flowering plants after 2 years of seed inoculation. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1175-8643 |
| 出版地 | ABINGDON |
| WOS关键词 | MYCORRHIZAL SPECIFICITY ; TERRESTRIAL ORCHIDS ; MOLECULAR VARIATION ; FUNGI ; GENUS ; RHIZOCTONIA ; ENDOPHYTES ; SEQUENCES ; ROOTS |
| 语种 | 英语 |
| 出版者 | TAYLOR & FRANCIS LTD |
| WOS记录号 | WOS:000425492000007 |
| 资助机构 | Wellington Botanical Society ; San Diego County Orchid Society (USA) ; Australian Orchid Foundation [287/2012] ; Te Papa MSc Scholarship in Plant Molecular Systematics |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20419]  |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.[Frericks, Jonathan; Lehnebach, Carlos A.] Museum New Zealand Te Papa Tongarewa, Wellington, New Zealand 2.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 3.Victoria Univ Wellington, Sch Biol Sci, Wellington, New Zealand |
| 推荐引用方式 GB/T 7714 | Frericks, Jonathan,Munkacsi, Andrew,Ritchie, Peter,et al. Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae[J]. NEW ZEALAND JOURNAL OF BOTANY,2018,56(1):91-108. |
| APA | Frericks, Jonathan,Munkacsi, Andrew,Ritchie, Peter,Luo, Yi-Bo,&Lehnebach, Carlos A..(2018).Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae.NEW ZEALAND JOURNAL OF BOTANY,56(1),91-108. |
| MLA | Frericks, Jonathan,et al."Phylogenetic affinities and in vitro seed germination of the threatened New Zealand orchid Spiranthes novae-zelandiae".NEW ZEALAND JOURNAL OF BOTANY 56.1(2018):91-108. |
入库方式： OAI收割
来源：植物研究所
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