A primer to the use of herbarium specimens in plant phylogenetics
文献类型：期刊论文
| 作者 | Wang, Wei2 |
| 刊名 | BOTANY LETTERS
 |
| 出版日期 | 2018 |
| 卷号 | 165期号:3-4页码:404-408 |
| 关键词 | Contamination DNA extraction herbarium specimen PCR amplification molecular phylogeny |
| ISSN号 | 2381-8107 |
| DOI | 10.1080/23818107.2018.1438311 |
| 文献子类 | Article |
| 英文摘要 | As an important DNA source, herbarium specimens have been widely used in plant phylogenetics due to their easy accessibility and richness. However, contamination is a constant risk owing to low quality and/or quantity of DNA extracted from old samples. The inclusion of contaminant sequences can mislead phylogenetic reconstruction, and thereby affect subsequent analyses, such as divergence time estimates and biogeographic inferences. To employ appropriately herbarium specimens in plant phylogenetics, we outline here several suggestions regarding DNA extraction, PCR amplification and sequencing. We hope that these suggestions will serve as a guide for researchers using herbarium specimens in plant phylogenetics and help them identify from the early steps potential contaminant sequences from other plants or other organisms. |
| 学科主题 | Plant Sciences |
| 出版地 | ABINGDON |
| 电子版国际标准刊号 | 2381-8115 |
| WOS关键词 | MOLECULAR PHYLOGENY ; DNA ; CLASSIFICATION ; MITOCHONDRIAL ; EXTRACTION ; SEQUENCE ; MENISPERMACEAE ; RANUNCULACEAE ; AMPLIFICATION ; BIOGEOGRAPHY |
| 语种 | 英语 |
| WOS记录号 | WOS:000457064300010 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770231, 31470315] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB954100] ; Youth Innovation Promotion Association Foundation of CAS ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDPB0203] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20535]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wei. A primer to the use of herbarium specimens in plant phylogenetics[J]. BOTANY LETTERS,2018,165(3-4):404-408. |
| APA | Wang, Wei.(2018).A primer to the use of herbarium specimens in plant phylogenetics.BOTANY LETTERS,165(3-4),404-408. |
| MLA | Wang, Wei."A primer to the use of herbarium specimens in plant phylogenetics".BOTANY LETTERS 165.3-4(2018):404-408. |
入库方式： OAI收割
来源：植物研究所
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