Evolution and Function of the Populus SABATH Family Reveal That a Single Amino Acid Change Results in a Substrate Switch
文献类型：期刊论文
| 作者 | Han, Xue-Min; Yang, Qi; Liu, Yan-Jing; Yang, Zhi-Ling; Wang, Xiao-Ru; Zeng, Qing-Yin; Yang, Hai-Ling |
| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 59期号:2页码:392-403 |
| 关键词 | Enzyme activity Enzyme family Expression divergence Molecular evolution Populus trichocarpa Substrate switch |
| ISSN号 | 0032-0781 |
| DOI | 10.1093/pcp/pcx198 |
| 文献子类 | Article |
| 英文摘要 | Evolutionary mechanisms of substrate specificities of enzyme families remain poorly understood. Plant SABATH methyltransferases catalyze methylation of the carboxyl group of various low molecular weight metabolites. Investigation of the functional diversification of the SABATH family in plants could shed light on the evolution of substrate specificities in this enzyme family. Previous studies identified 28 SABATH genes from the Populus trichocarpa genome. In this study, we re-annotated the Populus SABATH gene family, and performed molecular evolution, gene expression and biochemical analyses of this large gene family. Twenty-eight Populus SABATH genes were divided into three classes with distinct divergences in their gene structure, expression responses to abiotic stressors and enzymatic properties of encoded proteins. Populus class I SABATH proteins converted IAA to methyl-IAA, class II SABATH proteins converted benzoic acid (BA) and salicylic acid (SA) to methyl-BA and methyl-SA, while class III SABATH proteins converted farnesoic acid (FA) to methyl-FA. For Populus class II SABATH proteins, both forward and reverse mutagenesis studies showed that a single amino acid switch between PtSABATH4 and PtSABATH24 resulted in substrate switch. Our findings provide new insights into the evolution of substrate specificities of enzyme families. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1471-9053 |
| WOS关键词 | SYSTEMIC ACQUIRED-RESISTANCE ; JUVENILE-HORMONE-III ; ARABIDOPSIS-THALIANA ; SALICYLIC-ACID ; METHYL SALICYLATE ; INDOLE-3-ACETIC-ACID METHYLTRANSFERASE ; BIOCHEMICAL-CHARACTERIZATION ; MAXIMUM-LIKELIHOOD ; BLACK COTTONWOOD ; GENE FAMILY |
| 语种 | 英语 |
| WOS记录号 | WOS:000425507200015 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 31570664, 31425006] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20544]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Wang, Xiao-Ru 2.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 3.[Yang, Qi 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 6.[Han, Xue-Min 7.Yang, Zhi-Ling |
| 推荐引用方式 GB/T 7714 | Han, Xue-Min,Yang, Qi,Liu, Yan-Jing,et al. Evolution and Function of the Populus SABATH Family Reveal That a Single Amino Acid Change Results in a Substrate Switch[J]. PLANT AND CELL PHYSIOLOGY,2018,59(2):392-403. |
| APA | Han, Xue-Min.,Yang, Qi.,Liu, Yan-Jing.,Yang, Zhi-Ling.,Wang, Xiao-Ru.,...&Yang, Hai-Ling.(2018).Evolution and Function of the Populus SABATH Family Reveal That a Single Amino Acid Change Results in a Substrate Switch.PLANT AND CELL PHYSIOLOGY,59(2),392-403. |
| MLA | Han, Xue-Min,et al."Evolution and Function of the Populus SABATH Family Reveal That a Single Amino Acid Change Results in a Substrate Switch".PLANT AND CELL PHYSIOLOGY 59.2(2018):392-403. |
入库方式： OAI收割
来源：植物研究所
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