Diatom DNA barcodes for forensic discrimination of drowning incidents
文献类型：期刊论文
| 作者 | Liu, Mengyan4; Zhao, Yi4; Sun, Yuzhe1,3; Wu, Ping1,3; Zhou, Shiliang1,3; Ren, Liang |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2020 |
| 卷号 | 367期号:17 |
| 关键词 | 18S diatom DNA barcode primer rbcL |
| ISSN号 | 0378-1097 |
| DOI | 10.1093/femsle/fnaa145 |
| 文献子类 | Article |
| 英文摘要 | The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.In this study we tested 23 pairs of primers, including two new primer pairs, Baci18S (V4 of 18S) and BacirbcL (central region of rbcL), for amplifying fragments of 16S/18S, 23S/28S, COI, ITS and rbcL. A total of five pairs of primers performed satisfactory for diatoms. We used three of them, 18S605 (V2 + V3 of 18S), Baci18S and BacirbcL, to barcode four water samples using next generation sequencing platform. The results showed that these primers worked well for NGS metabarcoding of diatoms. We suggest that 18S605, Baci18S and BacirbcL be barcodes of diatoms and the corresponding primer pairs be used. Considering a quite high proportion of sequences deposited in GenBank were mislabeled, the most urgent task for DNA barcoding of diatoms is to create standard sequences using correctly identified specimens, ideally type specimens. |
| 学科主题 | Microbiology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1574-6968 |
| WOS关键词 | IDENTIFICATION ; SOFTWARE ; RBCL |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000602578400008 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21487]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Xiangshan St, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Beijing Publ Secur Bur, Forens Judicial Appraisal Ctr, 1 Longgang Beijing Rd, Beijing 100192, Peoples R China 4.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Forens Med, 13 HangkongRd, Wuhan 430030, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Mengyan,Zhao, Yi,Sun, Yuzhe,et al. Diatom DNA barcodes for forensic discrimination of drowning incidents[J]. FEMS MICROBIOLOGY LETTERS,2020,367(17). |
| APA | Liu, Mengyan,Zhao, Yi,Sun, Yuzhe,Wu, Ping,Zhou, Shiliang,&Ren, Liang.(2020).Diatom DNA barcodes for forensic discrimination of drowning incidents.FEMS MICROBIOLOGY LETTERS,367(17). |
| MLA | Liu, Mengyan,et al."Diatom DNA barcodes for forensic discrimination of drowning incidents".FEMS MICROBIOLOGY LETTERS 367.17(2020). |
入库方式： OAI收割
来源：植物研究所
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