Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition
文献类型：期刊论文
| 作者 | Sang, Qing; Pajoro, Alice; Sun, Hequan; Song, Baoxing; Yang, Xia3; Stolze, Sara C.; Andres, Fernando1; Schneeberger, Korbinian; Nakagami, Hirofumi; Coupland, George |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2020 |
| 卷号 | 32期号:5页码:1479-1500 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.19.00992 |
| 文献子类 | Article |
| 英文摘要 | Several pathways conferring environmental flowering responses in Arabidopsis (Arabidopsis thaliana) converge on developmental processes that mediate the floral transition in the shoot apical meristem. Many characterized mutations disrupt these environmental responses, but downstream developmental processes have been more refractory to mutagenesis. Here, we constructed a quintuple mutant impaired in several environmental pathways and showed that it possesses severely reduced flowering responses to changes in photoperiod and ambient temperature. RNA-sequencing (RNA-seq) analysis of the quintuple mutant showed that the expression of genes encoding gibberellin biosynthesis enzymes and transcription factors involved in the age pathway correlates with flowering. Mutagenesis of the quintuple mutant generated two late-flowering mutants, quintuple ems1 (qem1) and qem2. The mutated genes were identified by isogenic mapping and transgenic complementation. The qem1 mutant is an allele of the gibberellin 20-oxidase gene ga20ox2, confirming the importance of gibberellin for flowering in the absence of environmental responses. By contrast, qem2 is impaired in CHROMATIN REMODELING4 (CHR4), which has not been genetically implicated in floral induction. Using co-immunoprecipitation, RNA-seq, and chromatin immunoprecipitation sequencing, we show that CHR4 interacts with transcription factors involved in floral meristem identity and affects the expression of key floral regulators. Therefore, CHR4 mediates the response to endogenous flowering pathways in the inflorescence meristem to promote floral identity. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1531-298X |
| WOS关键词 | CHROMATIN-REMODELING FACTOR ; LATE-FLOWERING MUTANTS ; DOMAIN PROTEIN ; FT PROTEIN ; AMBIENT-TEMPERATURE ; MERISTEM IDENTITY ; SHOOT DEVELOPMENT ; ORGAN IDENTITY ; LIFE-CYCLE ; BOX GENE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000545741500021 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | European Molecular Biology OrganizationEuropean Molecular Biology Organization (EMBO) [AFL-2017-74] ; China Scholarship CouncilChina Scholarship Council [201804910196] ; Deutsche Forschungsgemeinschaft under Germany's Excellence StrategyGerman Research Foundation (DFG) [DFGEXC 2048/1, 390686111] ; Max Planck SocietyMax Planck SocietyFoundation CELLEX |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21696]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Montpellier, SupAgro, Ctr Cooperat Int Rech Agron Dev, INRA,Ameliorat Genet & Adaptat Plantes Mediterran, F-34398 Montpellier, France 3.Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany |
| 推荐引用方式 GB/T 7714 | Sang, Qing,Pajoro, Alice,Sun, Hequan,et al. Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition[J]. PLANT CELL,2020,32(5):1479-1500. |
| APA | Sang, Qing.,Pajoro, Alice.,Sun, Hequan.,Song, Baoxing.,Yang, Xia.,...&Coupland, George.(2020).Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition.PLANT CELL,32(5),1479-1500. |
| MLA | Sang, Qing,et al."Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition".PLANT CELL 32.5(2020):1479-1500. |
入库方式： OAI收割
来源：植物研究所
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