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Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals

文献类型：期刊论文

作者Jiang, Yongchao1; Wang, Meimei1; Zhang, Rui; Xie, Jinghe1; Duan, Xiaoshan; Shan, Hongyan; Xu, Guixia; Kong, Hongzhi1
刊名NEW PHYTOLOGIST
出版日期2020
卷号227期号:4页码:1235-1248
关键词Aquilegia binding sites ChIP-seq floral organ identity genes petals target genes
ISSN号0028-646X
DOI10.1111/nph.16601
文献子类Article
英文摘要Identification and comparison of the conserved and variable downstream genes of floral organ identity regulators are critical to understanding the mechanisms underlying the commonalities and peculiarities of floral organs. Yet, because of the lack of studies in nonmodel species, a general picture of the regulatory evolution between floral organ identity genes and their targets is still lacking. Here, by conducting extensive chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), electrophoretic mobility shift assay and bioinformatic analyses, we identify and predict the target genes of a petal identity gene, AqAPETALA3-3 (AqAP3-3), in Aquilegia coerulea (Ranunculaceae) and compare them with those of its counterpart in Arabidopsis thaliana, AP3. In total, 7049 direct target genes are identified for AqAP3-3, of which 2394 are highly confident and 1085 are shared with AP3. Gene Ontology enrichment analyses further indicate that conserved targets are largely involved in the formation of identity-related features, whereas nonconserved targets are mostly required for the formation of species-specific features. These results not only help understand the molecular bases of the conserved and nonconserved features of petals, but also pave the way to studying the regulatory evolution between floral organ identity genes and their targets.
学科主题Plant Sciences
出版地HOBOKEN
电子版国际标准刊号1469-8137
WOS关键词MADS-BOX GENES ; FLORAL ORGAN IDENTITY ; TRANSCRIPTION FACTOR ; CHIP-SEQ ; ARABIDOPSIS PETAL ; HOMEOBOX GENES ; DNA-BINDING ; EVOLUTION ; APETALA3 ; PROTEIN
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000533141000001
出版者WILEY
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930008] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB27010304]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/21761]  
专题植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文
作者单位1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Yongchao,Wang, Meimei,Zhang, Rui,et al. Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals[J]. NEW PHYTOLOGIST,2020,227(4):1235-1248.
APA Jiang, Yongchao.,Wang, Meimei.,Zhang, Rui.,Xie, Jinghe.,Duan, Xiaoshan.,...&Kong, Hongzhi.(2020).Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals.NEW PHYTOLOGIST,227(4),1235-1248.
MLA Jiang, Yongchao,et al."Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals".NEW PHYTOLOGIST 227.4(2020):1235-1248.

入库方式： OAI收割

来源：植物研究所

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