Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica
文献类型：期刊论文
| 作者 | Zhuge, Xiang-Lin; Xu, Hui1; Xiu, Zhi-Jing; Yang, Hai-Ling |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2020 |
| 卷号 | 11 |
| 关键词 | glutathione S-transferase gene expression enzyme activity kinetic analysis salix babylonica |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2020.00364 |
| 文献子类 | Article |
| 英文摘要 | Glutathione S-transferases (GSTs) are ubiquitous enzymes that are encoded by a large gene family, and they contribute to the detoxification of endogenous or xenobiotic compounds and oxidative stress metabolism in plants. Although the GSTs gene family has been reported in many land plants, our knowledge of the evolution and function of the willow GSTs is still limited. In this study, 22 full-length GST genes were cloned from Salix babylonica and divided into three classes based on the conserved domain analysis, phylogenetic tree and gene structure: tau, phi and DHAR. The tissue-specific expression patterns were substantially different among the tau and phi GSTs. The Salix GST proteins showed functional divergences in the substrate specificities, substrate activities and kinetic characteristics. The site-directed mutagenesis studies revealed that a single amino acid mutation (Ile/Val53 -> Thr53) resulted in the lowest activity of SbGSTU7 among the Salix GSTs. These results suggest that non-synonymous substitution of an amino acid at the putative glutathione-binding site may play an important role in the divergence of enzymatic functions of Salix GST family. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | SUPERGENE FAMILY ; GENE FAMILY ; TOLERANCE ; DIVERGENCE ; EXPRESSION ; EVOLUTION ; LIGHT |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000528744500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 31570664] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21790]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhuge, Xiang-Lin,Xu, Hui,Xiu, Zhi-Jing,et al. Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica[J]. FRONTIERS IN PLANT SCIENCE,2020,11. |
| APA | Zhuge, Xiang-Lin,Xu, Hui,Xiu, Zhi-Jing,&Yang, Hai-Ling.(2020).Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica.FRONTIERS IN PLANT SCIENCE,11. |
| MLA | Zhuge, Xiang-Lin,et al."Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica".FRONTIERS IN PLANT SCIENCE 11(2020). |
入库方式： OAI收割
来源：植物研究所
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