Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa
文献类型：期刊论文
| 作者 | Liu, Hai-Jing; Wang, Xin; Yang, Zhi-Ling; Ren, Lin-Ling; Qian, Ting-Ting |
| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2020 |
| 卷号 | 294 |
| 关键词 | Glutathione reductase Subcellular localization Expression pattern Biochemical characteristics Populus trichocarpa |
| ISSN号 | 0168-9452 |
| DOI | 10.1016/j.plantsci.2020.110459 |
| 文献子类 | Article |
| 英文摘要 | Glutathione reductase (GR; EC 1.6.4.2) is a key NADPH-dependent flavo-protein oxidoreductase which can catalyze the oxidized glutathione (GSSG) to reduced glutathione (GSH) to protect plant cells from oxidative damage induced by Reactive oxygen species (ROS) burst. To investigate the biochemical characteristics and functional divergence of Populus GR family, three GR genes (PtGR1.1/1.2/2) were cloned from Populus trichocarpa and their biochemical characteristics were analyzed in this study. All the three genes were expressed in root, stem, leaf and bud, and the expression of PtGR genes were general upregulated under salicylic acid and alamethicin treatment. PtGR1.1 and PtGR1.2 were localized in cytoplasm, while PtGR2 was in chloroplast. The three PtGR proteins showed different enzymatic activities, apparent kinetic characteristic and thermal stability profiles. However, they have similar bivalent metal ions (Cu2+, Cd2+, Zn2+ and Pb2+) sensitivity and optimum pH profiles. Our study sheds light on a comprehensive information of glutathione reductase family in P. trichocarpa, and proved PtGR genes play critical roles when suffering different stresses. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CLARE |
| WOS关键词 | INDUCED OXIDATIVE STRESS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; ENZYME-ACTIVITY ; PLANT ; OXYGEN ; DETOXIFICATION ; EVOLUTION ; ASCORBATE ; GENOME |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000528164300014 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21794]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Yang, Zhi-Ling 5.Wang, Xin 6.[Liu, Hai-Jing 7.Inner Mongolia Univ, Sch Life Sci, Key Lab Herbage & Endem Crop Biotechnol, Hohhot 010070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hai-Jing,Wang, Xin,Yang, Zhi-Ling,et al. Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa[J]. PLANT SCIENCE,2020,294. |
| APA | Liu, Hai-Jing,Wang, Xin,Yang, Zhi-Ling,Ren, Lin-Ling,&Qian, Ting-Ting.(2020).Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa.PLANT SCIENCE,294. |
| MLA | Liu, Hai-Jing,et al."Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa".PLANT SCIENCE 294(2020). |
入库方式： OAI收割
来源：植物研究所
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