Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice
文献类型：期刊论文
| 作者 | Kim, Hyo-Jung; Joe, Hae-In; Zhang, Zhiyun5; Lee, Sang Woo1; Lee, Kyou-Young; Kook, Yoon-Bum; An, Hyo-Jin |
| 刊名 | MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 119页码:123-131 |
| 关键词 | Acalypha australis L. Anti-inflammation Lipopolysaccharide NF-kappa B RAW 264.7 macrophages Septic shock |
| ISSN号 | 0161-5890 |
| DOI | 10.1016/j.molimm.2020.01.010 |
| 文献子类 | Article |
| 英文摘要 | We evaluated the anti-inflammatory activity of methanol extracts of Chinese medicinal plants from Beijing and determined which extract was the most effective. We found the methanol extract of Acalypha auswalis L. (AAL) to be the most effective. AAL has been used for clearing heat, toxic material, and hemostasia in Chinese medicine. Although these uses are closely related to inflammation, the anti-inflammatory effect of AAL has not yet been described and its underlying mechanism remains unclear. Therefore, we aimed to identify anti-inflammatory effect of AAL and its underlying mechanism in vitro and in vivo. In RAW 264.7 macrophages, cytotoxicity was evaluated by MTT assay and nitric oxide (NO) was measured with Griess reagent. To confirm the production of pro-inflammatory cytokines and its mRNA expression, enzyme immunoassay (ETA) and quantitative real-time PCR (qRT-PCR) were performed. Further, protein expression was analyzed by western blotting. Septic shock was induced by intraperitoneal injection of LPS (25 mg/kg) in mice. One hour before LPS injection, AAL (25 and 50 mg/kg) was administered orally. In LPS-stimulated macrophages, AAL inhibited NO production at concentrations without cytotoxicity. Additionally, AAL reduced not only inducible nitric oxide synthase (iNOS) expression but the production of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) by attenuating nuclear factor-kappa B (NF-kappa B)-related proteins (NF-kappa B p65, phosphorylation of inhibitor kappa B-alpha; p-I kappa B-alpha, phosphorylation of inhibitor kappa B kinase-alpha/beta; p-Ikk-alpha/beta). Moreover, AAL enhanced the survival rate of mice through the inhibition of iNOS expression and IL-6 and interleukin-1 beta (IL-1 beta) production in LPS-induced septic mice. Furthermore, AAL also reduced the expression of NF-kappa B-related proteins. These finding suggest that AAL is related to the modulation of inflammatory reactions by blocking NF-kappa B activation in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice. |
| 学科主题 | Biochemistry & Molecular Biology ; Immunology |
| 出版地 | OXFORD |
| WOS关键词 | INFLAMMATORY MEDIATORS ; ENDOTOXIC-SHOCK ; NITRIC-OXIDE ; INACTIVATION ; INHIBITION ; ACTIVATION ; RESPONSES ; PROTECTS ; LINN. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000517657900015 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Research Foundation of Korea (NRF) - the Korea government (MSIP ; Ministry of Science, ICT & Future Planning) [NRF-2018R1C1B5041014] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21888]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Sangji Univ, Coll Korean Med, Dept Pharmacol, 83 Sangjidae Gil, Wonju 26339, Gangwon Do, South Korea 3.Sangji Univ, Coll Korean Med, Dept Korean Ophthalmol & Otolaryngol & Dermatol, 83 Sangjidae Gil, Wonju 26339, Gangwon Do, South Korea 4.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea 5.Sangji Univ, Coll Korean Med, Dept Prescript, 83 Sangjidae Gil, Wonju 26339, Gangwon Do, South Korea |
| 推荐引用方式 GB/T 7714 | Kim, Hyo-Jung,Joe, Hae-In,Zhang, Zhiyun,et al. Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice[J]. MOLECULAR IMMUNOLOGY,2020,119:123-131. |
| APA | Kim, Hyo-Jung.,Joe, Hae-In.,Zhang, Zhiyun.,Lee, Sang Woo.,Lee, Kyou-Young.,...&An, Hyo-Jin.(2020).Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice.MOLECULAR IMMUNOLOGY,119,123-131. |
| MLA | Kim, Hyo-Jung,et al."Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice".MOLECULAR IMMUNOLOGY 119(2020):123-131. |
入库方式： OAI收割
来源：植物研究所
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