A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis
文献类型：期刊论文
| 作者 | Li, Wei5; Zhang, Fengxia5; Wu, Ranran5; Jia, Lijia1,4; Li, Guosheng3,5; Guo, Yalong2 ; Liu, Cuimin1,4; Wang, Guodong5
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| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 174期号:3页码:1492-1504 |
| ISSN号 | 0032-0889 |
| DOI | 10.1002/2017JG003968 |
| 文献子类 | Article |
| 英文摘要 | The Preiss-Handler pathway, which salvages nicotinate (NA) for NAD synthesis, is an indispensable biochemical pathway in land plants. Various NA conjugations (mainly methylation and glycosylation) have been detected and have long been proposed for NA detoxification in plants. Previously, we demonstrated that NA O-glucosylation functions as a mobilizable storage form for NAD biosynthesis in the Brassicaceae. However, little is known about the functions of other NA conjugations in plants. In this study, we first found that N-methylnicotinate is a ubiquitous NA conjugation in land plants. Furthermore, we functionally identified a novel methyltransferase (At3g53140; NANMT), which is mainly responsible for N-methylnicotinate formation, from Arabidopsis (Arabidopsis thaliana). We also established that trigonelline is a detoxification form of endogenous NA in plants. Combined phylogenetic analysis and enzymatic assays revealed that NA N-methylation activity was likely derived from the duplication and subfunctionalization of an ancestral caffeic acid O-methyltransferase (COMT) gene in the course of land plant evolution. COMT enzymes, which function in S-lignin biosynthesis, also have weak NANMT activity. Our data suggest that NA detoxification conferred by NANMT and COMT might have facilitated the retention of the Preiss-Handler pathway in land plants. |
| 学科主题 | Environmental Sciences & Ecology ; Geology |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | ACID O-METHYLTRANSFERASE ; CONVERGENT EVOLUTION ; DIPHOSPHOPYRIDINE NUCLEOTIDE ; SUBSTRATE-SPECIFICITY ; TRIGONELLINE ; GERMINATION ; COFFEE ; NAD ; EXPRESSION ; COMPONENTS |
| 语种 | 英语 |
| WOS记录号 | WOS:000418086800025 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Program on Key Basic Research Projects (973 Program)National Basic Research Program of China [2013CB127000] ; National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270336] ; State Key Laboratory of Plant Genomics of China [2014b0227-05] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22028]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Ctr Mol Agrobiol, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 4.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 5.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 6.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wei,Zhang, Fengxia,Wu, Ranran,et al. A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis[J]. PLANT PHYSIOLOGY,2017,174(3):1492-1504. |
| APA | Li, Wei.,Zhang, Fengxia.,Wu, Ranran.,Jia, Lijia.,Li, Guosheng.,...&Wang, Guodong.(2017).A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis.PLANT PHYSIOLOGY,174(3),1492-1504. |
| MLA | Li, Wei,et al."A Novel N-Methyltransferase in Arabidopsis Appears to Feed a Conserved Pathway for Nicotinate Detoxification among Land Plants and Is Associated with Lignin Biosynthesis".PLANT PHYSIOLOGY 174.3(2017):1492-1504. |
入库方式： OAI收割
来源：植物研究所
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