Identification and characterization of Rubisco activase genes in Oryza punctata
文献类型：期刊论文
| 作者 | Xu, Si; Qin, Zong-Yan; Gong, Pi-Chang; Dong, Qian-Li; Bao, Ying2 |
| 刊名 | JOURNAL OF SYSTEMATICS AND EVOLUTION
 |
| 出版日期 | 2017 |
| 卷号 | 55期号:3页码:200-207 |
| 关键词 | Rubisco activase alternative splicing wild rice expression divergence protein interaction |
| ISSN号 | 1674-4918 |
| DOI | 10.1080/13658816.2016.1195502 |
| 文献子类 | Article |
| 英文摘要 | Rubisco activase (Rca), a specific chaperone, catalyzes the in vivo activation of Rubisco, and thus plays a major role in plant photosynthesis. Although the genes encoding Rubisco activase have been studied in many model or economic plants, few studies have analyzed their homologs in plants of closely related crop species. In this study, an Rca gene was identified and characterized in a wild relative of rice, Oryza punctata Kotschy ex Steud. The gene was 2747 bp long and possessed six exons and five introns with 47% GC content. Furthermore, cDNA sequencing produced two transcripts, RcaL and RcaS, that differed in the sequence by an inclusion of 99 bp at the carboxy terminus of RcaL. Sequence comparison between the two transcripts and the genomic DNA showed that there was a 20-bp alternative splicing event that occurred at the fifth intron of the gene leading to the synthesis of a short polypeptide. The leaf transcriptome analysis showed that RcaS had a higher expression level than that of RcaL in a normal growth environment. In addition, the yeast two-hybrid assays showed the small isoform of Rubisco activase in close contact with the large subunit of the Rubisco in this species, supporting the side-on binding model of interaction between two proteins. This study broadens our understanding of the molecular characteristics of some essential genes in photosynthesis. |
| 学科主题 | Computer Science ; Geography ; Physical Geography ; Information Science & Library Science |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1759-6831 |
| WOS关键词 | RIBULOSE 1,5-BISPHOSPHATE ; CARBOXYLASE/OXYGENASE ACTIVASE ; EXPRESSION ; PLANTS ; PHOTOSYNTHESIS ; SUBSTITUTION ; ALIGNMENT ; ISOFORMS ; ENZYMES ; MAIZE |
| 语种 | 英语 |
| WOS记录号 | WOS:000387268600010 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570218] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22074]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Northeast Normal Univ, Key Lab Mol Epigenet, Minist Educ, Changchun 130024, Peoples R China 2.Qin, Zong-Yan 3.Qufu Normal Univ, Sch Life Sci, Qufu 273165, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.[Xu, Si |
| 推荐引用方式 GB/T 7714 | Xu, Si,Qin, Zong-Yan,Gong, Pi-Chang,et al. Identification and characterization of Rubisco activase genes in Oryza punctata[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2017,55(3):200-207. |
| APA | Xu, Si,Qin, Zong-Yan,Gong, Pi-Chang,Dong, Qian-Li,&Bao, Ying.(2017).Identification and characterization of Rubisco activase genes in Oryza punctata.JOURNAL OF SYSTEMATICS AND EVOLUTION,55(3),200-207. |
| MLA | Xu, Si,et al."Identification and characterization of Rubisco activase genes in Oryza punctata".JOURNAL OF SYSTEMATICS AND EVOLUTION 55.3(2017):200-207. |
入库方式： OAI收割
来源：植物研究所
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