Characterization of and isolation methods for plant leaf nanovesicles and small extracellular vesicles
文献类型：期刊论文
| 作者 | Liu, Yuan; Wu, Sherry2; Koo, Yeonjong1; Yang, An5; Dai, Yanwan; Khant, Htet3,4; Osman, Samantha R.; Chowdhury, Mamur; Wei, Haichao7; Li, Yang |
| 刊名 | NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
 |
| 出版日期 | 2020 |
| 卷号 | 29 |
| 关键词 | Cancer cell uptake Isolation method Leaf nanovesicles Plant sEVs Plant sEV and nanovesicle proteome Therapeutic delivery |
| ISSN号 | 1549-9634 |
| DOI | 10.1016/j.nano.2020.102271 |
| 文献子类 | Article |
| 英文摘要 | Mammalian small extracellular vesicles (sEVs) can deliver diverse molecules to target cells. However, they are difficult to obtain in large quantities and can activate host immune responses. Plant-derived vesicles may help to overcome these challenges. We optimized isolation methods for two types of plant vesicles, nanovesicles from disrupted leaf and sEVs from the extracellular apoplastic space of Arabidopsis thaliana. Both preparations yielded intact vesicles of uniform size, and a mean membrane charge of approximately -25 mV. We also demonstrated applicability of these preparative methods using Brassicaceae vegetables. Proteomic analysis of a subset of vesicles with a density of 1.1-1.19 g mL(-1) sheds light on the likely cellular origin and complexity of the vesicles. Both leaf nanovesicles and sEVs were taken up by cancer cells, with sEVs showing an approximately three-fold higher efficiency compared to leaf nanovesicles. These results support the potential of plant-derived vesicles as vehicles for therapeutic delivery. (c) 2020 Elsevier Inc. All rights reserved. |
| 学科主题 | Nanoscience & Nanotechnology ; Medicine, Research & Experimental |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1549-9642 |
| WOS关键词 | DRUG-DELIVERY-SYSTEMS ; EXOSOMES ; PROTEIN ; CELLS ; NANOPARTICLES ; COMMUNICATION ; TRAFFICKING ; SECRETION ; TRANSPORT ; COLITIS |
| WOS研究方向 | Science & Technology - Other Topics ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000573096700017 |
| 出版者 | ELSEVIER |
| 资助机构 | National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R35 CA209904, P50 CA217685, P50 CA098258, CA016672] ; Blanton-Davis Ovarian Cancer Research Program ; American Cancer Society Research Professor AwardAmerican Cancer Society ; FNLCR [HHSN261200800001E] ; Frank McGraw Memorial Chair in Cancer Research |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21574]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia 2.Leidos Biomed Inc, Frederick Natl Lab Canc Res, Canc Res Technol Program, Frederick, MD USA 3.NCI, Ctr Mol Microscopy, Ctr Canc Res, Frederick, MD 21701 USA 4.Univ Texas Hlth Sci Ctr Houston, McGovern Med Sch, Vivian L Smith Dept Neurosurg, Houston, TX 77030 USA 5.Rice Univ, BioSci, Houston, TX USA 6.[Liu, Yuan; Wu, Sherry; Chowdhury, Mamur; Li, Yang; Court, Karem; Wen, Yunfei; Dasari, Santosh K.; Sood, Anil K.] Univ Texas MD Anderson Canc Ctr, Dept Gynecol Oncol, Houston, TX 77030 USA 7.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 8.Chonnam Natl Univ, Dept Agr Chem, Gwangju, South Korea |
| 推荐引用方式 GB/T 7714 | Liu, Yuan,Wu, Sherry,Koo, Yeonjong,et al. Characterization of and isolation methods for plant leaf nanovesicles and small extracellular vesicles[J]. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,2020,29. |
| APA | Liu, Yuan.,Wu, Sherry.,Koo, Yeonjong.,Yang, An.,Dai, Yanwan.,...&Sood, Anil K..(2020).Characterization of and isolation methods for plant leaf nanovesicles and small extracellular vesicles.NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,29. |
| MLA | Liu, Yuan,et al."Characterization of and isolation methods for plant leaf nanovesicles and small extracellular vesicles".NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 29(2020). |
入库方式： OAI收割
来源：植物研究所
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