Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata
文献类型：期刊论文
| 作者 | Diao, Min2,4; Ren, Sulin2; Wang, Qiannan; Qian, Lichao1; Shen, Jiangfeng; Liu, Yule1; Huang, Shanjin |
| 刊名 | ELIFE
 |
| 出版日期 | 2018 |
| 卷号 | 7 |
| ISSN号 | 2050-084X |
| DOI | 10.7554/eLife.36316 |
| 文献子类 | Article |
| 英文摘要 | Here, we demonstrate that Arabidopsis thaliana Formin 2 (AtFH2) localizes to plasmodesmata (PD) through its transmembrane domain and is required for normal intercellular trafficking. Although loss-of-function atfh2 mutants have no overt developmental defect, PD's permeability and sensitivity to virus infection are increased in atfh2 plants. Interestingly, AtFH2 functions in a partially redundant manner with its closest homolog AtFH1, which also contains a PD localization signal. Strikingly, targeting of Class I formins to PD was also confirmed in rice, suggesting that the involvement of Class I formins in regulating actin dynamics at PD may be evolutionarily conserved in plants. In vitro biochemical analysis showed that AtFH2 fails to nucleate actin assembly but caps and stabilizes actin filaments. We also demonstrate that the interaction between AtFH2 and actin filaments is crucial for its function in vivo. These data allow us to propose that AtFH2 regulates PD's permeability by anchoring actin filaments to PD. |
| 学科主题 | Biology |
| 出版地 | CAMBRIDGE |
| WOS关键词 | CLASS-II FORMIN ; ARP2/3 COMPLEX ; PROTEIN ; CYTOSKELETON ; TOBACCO ; LOCALIZATION ; ORGANIZATION ; NUCLEATION ; MECHANISM ; MOVEMENT |
| 语种 | 英语 |
| WOS记录号 | WOS:000443934300001 |
| 出版者 | ELIFE SCIENCES PUBLICATIONS LTD |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471266, 31671390, 31121065] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20323]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing, Peoples R China 3.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, MOE Key Lab Bioinformat, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Diao, Min,Ren, Sulin,Wang, Qiannan,et al. Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata[J]. ELIFE,2018,7. |
| APA | Diao, Min.,Ren, Sulin.,Wang, Qiannan.,Qian, Lichao.,Shen, Jiangfeng.,...&Huang, Shanjin.(2018).Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata.ELIFE,7. |
| MLA | Diao, Min,et al."Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata".ELIFE 7(2018). |
入库方式： OAI收割
来源：植物研究所
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