Calcium imaging in Arabidopsis pollen cells using G-CaMP5
文献类型：期刊论文
| 作者 | Diao, Min2,3; Qu, Xiaolu2; Huang, Shanjin2 |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 60期号:9页码:897-906 |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.12642 |
| 文献子类 | Article |
| 英文摘要 | Calcium (Ca2+) signaling has been implicated in pollen germination and pollen tube growth. To date, however, we still know very little about how exactly Ca2+ signaling links to various physiological subcellular processes during pollen germination and pollen tube growth. Given that Ca2+ signaling is tightly related to the cytosolic concentration and dynamics of Ca2+, it is vital to trace the dynamic changes in Ca2+ levels in order to decode Ca2+ signaling. Here, we demonstrate that G-CaMP5 serves well as an indicator for monitoring cytosolic Ca2+ dynamics in pollen cells. Using this probe, we show that cytosolic Ca2+ changes dramatically during pollen germination, and, as reported previously, Ca2+ forms a tip-focused gradient in the pollen tube and undergoes oscillation in the tip region during pollen tube growth. In particular, using G-CaMP5 allowed us to capture the dynamic changes in the cytosolic Ca2+ concentration ([Ca2+](cyt)) in pollen tubes in response to various exogenous treatments. Our data suggest that G-CaMP5 is a suitable probe for monitoring the dynamics of [Ca2+](cyt) in pollen cells. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | GREEN FLUORESCENT PROTEIN ; TUBE TIP GROWTH ; PLASMA-MEMBRANE ; NEURAL ACTIVITY ; CA2+ CHANNELS ; GRADIENT ; CALMODULIN ; GERMINATION ; DYNAMICS ; INFLUX |
| 语种 | 英语 |
| WOS记录号 | WOS:000443808500012 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471266, 31671390] ; Tsinghua-Peking Joint Center for Life Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20423]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Diao, Min,Qu, Xiaolu,Huang, Shanjin. Calcium imaging in Arabidopsis pollen cells using G-CaMP5[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2018,60(9):897-906. |
| APA | Diao, Min,Qu, Xiaolu,&Huang, Shanjin.(2018).Calcium imaging in Arabidopsis pollen cells using G-CaMP5.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,60(9),897-906. |
| MLA | Diao, Min,et al."Calcium imaging in Arabidopsis pollen cells using G-CaMP5".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 60.9(2018):897-906. |
入库方式： OAI收割
来源：植物研究所
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