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Changes in the mitochondrial proteome of developing maize seed embryos

文献类型：期刊论文

作者Wang, Wei-Qing; Wang, Yue; Zhang, Qi2; Moller, Ian M.; Song, Song-Quan
刊名PHYSIOLOGIA PLANTARUM
出版日期2018
卷号163期号:4页码:552-572
ISSN号0031-9317
DOI10.1111/ppl.12725
文献子类Article
英文摘要Mitochondria are required for seed development, but little information is available about their function and role during this process. We isolated the mitochondria from developing maize (Zea mays L. cv. Nongda 108) embryos and investigated the mitochondrial membrane integrity and respiration as well as the mitochondrial proteome using two proteomic methods, the two-dimensional gel electrophoresis (2-DE) and sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH). Mitochondrial membrane integrity and respiration were maintained at a high level up to 21 days after pollination (DAP) and decreased thereafter, while total mitochondrial number, cytochrome c oxidase activity and respiration per embryo exhibited a bell-shaped change with peaks at 35-45 DAP. A total of 286 mitochondrial proteins changed in abundance during embryo development. During early stages of seed development (up to 21 DAP), proteins involved in energy production, basic metabolism, protein import and folding as well as removal of reactive oxygen species dominated, while during mid or late stages (35-70 DAP), some stress- and detoxification-related proteins increased in abundance. Our study, for the first time, depicted a relatively comprehensive map of energy production by mitochondria during embryo development. The results revealed that mitochondria were very active during the early stages of maize embryo development, while at the late stages of development, the mitochondria became more quiescent, but well-protected, presumably to ensure that the embryo passes through maturation, drying and long-term storage. These results advance our understanding of seed development at the organelle level.
学科主题Plant Sciences
出版地HOBOKEN
电子版国际标准刊号1399-3054
WOS关键词DESICCATION TOLERANCE ; ELECTRON-TRANSPORT ; PLANT-MITOCHONDRIA ; SUBCELLULAR-LOCALIZATION ; QUANTITATIVE PROTEOMICS ; PROTEINS ; ARABIDOPSIS ; STRESS ; GERMINATION ; BIOGENESIS
语种英语
WOS记录号WOS:000440004000009
出版者WILEY
资助机构National Sciences and Technology Support Program [2012BAC01B05] ; National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400292] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Sciences Visiting Professorship for senior international scientists
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/20497]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Moller, Ian M.] Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark
3.Guangdong Acad Agr Sci, Guangzhou 510640, Guangdong, Peoples R China
4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
5.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Wei-Qing,Wang, Yue,Zhang, Qi,et al. Changes in the mitochondrial proteome of developing maize seed embryos[J]. PHYSIOLOGIA PLANTARUM,2018,163(4):552-572.
APA Wang, Wei-Qing,Wang, Yue,Zhang, Qi,Moller, Ian M.,&Song, Song-Quan.(2018).Changes in the mitochondrial proteome of developing maize seed embryos.PHYSIOLOGIA PLANTARUM,163(4),552-572.
MLA Wang, Wei-Qing,et al."Changes in the mitochondrial proteome of developing maize seed embryos".PHYSIOLOGIA PLANTARUM 163.4(2018):552-572.

入库方式： OAI收割

来源：植物研究所

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