OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress
文献类型：期刊论文
| 作者 | Qian, Dandan; Chen, Guoqiang; Tian, Lihong; Qu, Le Qing |
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 178期号:1页码:402-412 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.18.00375 |
| 文献子类 | Article |
| 英文摘要 | Endoplasmic reticulum-associated protein degradation (ERAD) plays an important role in endoplasmic reticulum (ER) quality control. To date, little is known about the retrotranslocation machinery in the plant ERAD pathway. We obtained a DERLIN-like protein (OsDER1) through a SWATH-based quantitative proteomic analysis of ER membrane proteins extracted from ER-stressed rice (Oryza sativa) seeds. OsDER1, a homolog of yeast and mammal DER1, is localized in the ER and accumulates significantly under ER stress. Overexpression or suppression of OsDER1 in rice leads to activation of the unfolded protein response and hypersensitivity to ER stress, and suppression results in floury, shrunken seeds. In addition, the expression levels of polyubiquitinated proteins increased markedly in OsDER1 overexpression or suppression transgenic rice. Coimmunoprecipitation experiments demonstrated that OsDER1 interacted with OsHRD1, OsHRD3, and OsCDC48, the essential components of the canonical ERAD pathway. Furthermore, OsDER1 associated with the signal peptide peptidase, a homolog of a component of the alternative ERAD pathway identified recently in yeast and mammals. Our data suggest that OsDER1 is linked to the ERAD pathway. |
| 学科主题 | Plant Sciences |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | ENDOPLASMIC-RETICULUM STRESS ; MEMBRANE-PROTEIN ; RICE ENDOSPERM ; UBIQUITIN LIGASE ; QUALITY-CONTROL ; MISFOLDED GLYCOPROTEINS ; STORAGE PROTEINS ; GENE-EXPRESSION ; COMPLEX ; ARABIDOPSIS |
| 语种 | 英语 |
| WOS记录号 | WOS:000444004100031 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD0100500] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770271, 31171368, 31300212] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20539]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, Dandan,Chen, Guoqiang,Tian, Lihong,et al. OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress[J]. PLANT PHYSIOLOGY,2018,178(1):402-412. |
| APA | Qian, Dandan,Chen, Guoqiang,Tian, Lihong,&Qu, Le Qing.(2018).OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress.PLANT PHYSIOLOGY,178(1),402-412. |
| MLA | Qian, Dandan,et al."OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress".PLANT PHYSIOLOGY 178.1(2018):402-412. |
入库方式： OAI收割
来源：植物研究所
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