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Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen

文献类型：期刊论文

作者Han, Bing2,4; Yang, Ning2; Pu, Hai3; Wang, Tai2
刊名JOURNAL OF PROTEOME RESEARCH
出版日期2018
卷号17期号:4页码:1532-1546
关键词cell asymmetry filipin sterol-rich membrane microdomains label-free quantitative proteomics methyl-beta-cyclodextrin (M beta CD) polar growth pollen tube rice pollen
ISSN号1535-3893
DOI10.1021/acs.jproteome.7b00852
文献子类Article
英文摘要Cell polarity is essential for generating diverse cell functions. The underlying mechanisms of how a cell establishes, maintains, and changes its polarity are poorly understood. Recently, sterol-rich membrane microdomains are found to be associated with these processes. However, both its exact characteristics and importance are still unclear. Here we show microdomains change dynamically in developing and germinating rice pollen with selective enrichment in the aperture and the tip of newly born pollen tubes by use of the sterol-specific probe filipin. Using the sterol extraction sensitivities of microdomain proteins and quantitative proteomics, we identified 237 microdomain-associated proteins from 934 identified pollen detergent resistant membrane proteins. This proteome includes almost all of the known key regulators comprising the polar growth network, and it shows more similarity to front-back polarized HeLa cells than nonpolarized Arabidopsis suspension cells. We immunolocalize flotilin-like protein, a representative of these sterol-dependent proteins and directly visualize microdomains in pollen. These results indicate the presence of microdomains in pollen and preestablished cell polarity around the aperture during pollen maturation. Our findings reveal an atlas of the microdomain-associated proteome in pollen. This work provides useful resources and knowledge needed to further dissect the mechanisms for the establishment and maintenance of cell polarity.
学科主题Biochemical Research Methods
出版地WASHINGTON
电子版国际标准刊号1535-3907
WOS关键词DETERGENT-RESISTANT MEMBRANES ; EPITHELIAL-MESENCHYMAL TRANSITION ; MALE GAMETOPHYTE DEVELOPMENT ; RAFT-LIKE MICRODOMAINS ; PLASMA-MEMBRANE ; LIPID RAFTS ; TIP GROWTH ; TUBE GROWTH ; DEPENDENT ENDOCYTOSIS ; ARABIDOPSIS-THALIANA
语种英语
WOS记录号WOS:000429886600018
出版者AMER CHEMICAL SOC
资助机构Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2013CB945101] ; Chinese Academy of SciencesChinese Academy of Sciences [KSCX2-YW-N-026]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/20661]  
专题中科院植物分子生理学重点实验室
作者单位1.Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA
2.Bruker Dalton Inc China, Beijing 100081, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Han, Bing,Yang, Ning,Pu, Hai,et al. Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(4):1532-1546.
APA Han, Bing,Yang, Ning,Pu, Hai,&Wang, Tai.(2018).Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen.JOURNAL OF PROTEOME RESEARCH,17(4),1532-1546.
MLA Han, Bing,et al."Quantitative Proteomics and Cytology of Rice Pollen Sterol-Rich Membrane Domains Reveals Pre-established Cell Polarity Cues in Mature Pollen".JOURNAL OF PROTEOME RESEARCH 17.4(2018):1532-1546.

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来源：植物研究所

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