Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control
文献类型：期刊论文
| 作者 | Zhang, Qian-Qian; Li, Ying; Fu, Zhao-Ying; Liu, Xun-Biao; Yuan, Kai; Fang, Ying; Liu, Yan1; Li, Gang; Zhang, Xian-Sheng; Chong, Kang1 |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2018 |
| 卷号 | 95期号:1页码:150-167 |
| 关键词 | Arabidopsis cell cycling RPB1 stem cell niche |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.13939 |
| 文献子类 | Article |
| 英文摘要 | Plant meristem activity depends on accurate execution of transcriptional networks required for establishing optimum functioning of stem cell niches. An Arabidopsis mutant card1-1 (constitutive auxin response with DR5: GFP) that encodes a truncated RPB1 (RNA Polymerase II's largest subunit) with shortened C-terminal domain (CTD) was identified. Phosphorylation of the CTD repeats of RPB1 is coupled to transcription in eukaryotes. Here we uncover that the truncated CTD of RPB1 disturbed cell cycling and enlarged the size of shoot and root meristem. The defects in patterning of root stem cell niche in card1-1 indicates that intact CTD of RPB1 is necessary for fine-tuning the specific expression of genes responsible for cell-fate determination. The gene-edited plants with different CTD length of RPB1, created by CRISPR-CAS9 technology, confirmed that both the full length and the DK-rich tail of RPB1' s CTD play roles in the accurate transcription of CYCB1; 1 encoding a cell-cycle marker protein in root meristem and hence participate in maintaining root meristem size. Our experiment proves that the intact RPB1 CTD is necessary for stem cell niche maintenance, which is mediated by transcriptional regulation of cell cycling genes. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | RNA-POLYMERASE-II ; ROOT-MERISTEM SIZE ; C-TERMINAL DOMAIN ; GENE-EXPRESSION ; CTD ; AUXIN ; TRANSCRIPTION ; SHOOT ; GROWTH ; PLANT |
| 语种 | 英语 |
| WOS记录号 | WOS:000435446700013 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071066, 31370325] ; Taishan Scholar Program of Shandong Province |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20665]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qian-Qian,Li, Ying,Fu, Zhao-Ying,et al. Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control[J]. PLANT JOURNAL,2018,95(1):150-167. |
| APA | Zhang, Qian-Qian.,Li, Ying.,Fu, Zhao-Ying.,Liu, Xun-Biao.,Yuan, Kai.,...&Ge, Lei.(2018).Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control.PLANT JOURNAL,95(1),150-167. |
| MLA | Zhang, Qian-Qian,et al."Intact Arabidopsis RPB1 functions in stem cell niches maintenance and cell cycling control".PLANT JOURNAL 95.1(2018):150-167. |
入库方式： OAI收割
来源：植物研究所
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