Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus
文献类型：期刊论文
| 作者 | Liu, Jinyue1,4,5; Jiang, Wenbo1; Xia, Yaying1; Wang, Xuemin4; Shen, Guoan3; Pang, Yongzhen1,4 |
| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 59期号:1页码:128-141 |
| 关键词 | Genistein Isoflavonoids Isoflavonoid prenyl-transferase Lotus japonicus Wighteone |
| ISSN号 | 0032-0781 |
| DOI | 10.1093/pcp/pcx167 |
| 文献子类 | Article |
| 英文摘要 | Prenylated isoflavonoids have been found in several legume plants, and they possess various biological activities that play important roles in both plant defense and human health. However, it is still unknown whether prenylated isoflavonoids are present in the model legume plant Lotus japonicus. In the present study, we found that the prenylated isoflavonoid wighteone was produced in L. japonicus when leaf was supplemented with genistein. Furthermore, a novel prenyltransferase gene, LjG6DT, was identified, which shared high similarity with and was closely related to several known prenyltransferase genes involved in isoflavonoid biosynthesis. The recombinant LjG6DT protein expressed in yeast exhibited prenylation activity toward genistein as an exclusive substrate, which produced wighteone, a prenylated genistein at the C-6 position that occurs normally in legume plants. The LjG6DT-green fluorescent protein (GFP) fusion protein is targeted to plastids. The transcript level of LjG6DT is induced by glutathione, methyl jasmonate and salicylic acid, implying that LjG6DT is involved in stress response. Overexpression of LjG6DT in L. japonicus hairy roots led to increased accumulation of wighteone when genistein was supplied, indicating that LjG6DT is functional in vivo. Feeding assays with the upstream intermediate naringenin revealed that accumulation of wighteone in L. japonicus was dependent on genistein supplementation, and accumulation of wighteone is competed by genistein methylation. This study demonstrated that phytoalexin wighteone is inducibly produced in L. japonicus, and it provides new insight into the biosynthesis and accumulation of prenylated isoflavonoids in legume plants. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1471-9053 |
| WOS关键词 | MOLECULAR CHARACTERIZATION ; ISOFLAVONOID BIOSYNTHESIS ; PRENYLATED ISOFLAVONOIDS ; ENZYMATIC PRENYLATION ; PRENYLTRANSFERASE ; FLAVONOIDS ; IDENTIFICATION ; PROTEIN ; CLONING ; GLYCEOLLIN |
| 语种 | 英语 |
| WOS记录号 | WOS:000423660600019 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | International S&T Cooperation Program of China [2015DFR30570-3] ; Major State Basic Research and Development ProgramNational Basic Research Program of China [2014CB138703] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20689]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jinyue,Jiang, Wenbo,Xia, Yaying,et al. Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus[J]. PLANT AND CELL PHYSIOLOGY,2018,59(1):128-141. |
| APA | Liu, Jinyue,Jiang, Wenbo,Xia, Yaying,Wang, Xuemin,Shen, Guoan,&Pang, Yongzhen.(2018).Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus.PLANT AND CELL PHYSIOLOGY,59(1),128-141. |
| MLA | Liu, Jinyue,et al."Genistein-Specific G6DT Gene for the Inducible Production of Wighteone in Lotus japonicus".PLANT AND CELL PHYSIOLOGY 59.1(2018):128-141. |
入库方式： OAI收割
来源：植物研究所
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