Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains
文献类型：期刊论文
| 作者 | Liu, Jinxin; Wu, Xiaoba; Yao, Xuefeng; Yu, Ronald; Larkin, Philip J.; Liu, Chun-Ming |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2018 |
| 卷号 | 115期号:44页码:11327-11332 |
| ISSN号 | 0027-8424 |
| 关键词 | rice endosperm thick aleurone nutrition DNA demethylation |
| DOI | 10.1073/pnas.1806304115 |
| 文献子类 | Article |
| 英文摘要 | The rice endosperm, consisting of an outer single-cell layer aleurone and an inner starchy endosperm, is an important staple food for humans. While starchy endosperm stores mainly starch, the aleurone is rich in an array of proteins, vitamins, and minerals. To improve the nutritional value of rice, we screened for mutants with thickened aleurones using a half-seed assay and identified thick aleurone 2-1 (tat-1), in which the aleurone has 4.8 +/- 2.2 cell layers on average. Except for starch, the contents of all measured nutritional factors, including lipids, proteins, vitamins, minerals, and dietary fibers, were increased in tat-1 grains. Map-based cloning showed that TA2 encodes the DNA demethylase OsROS1. A point mutation in the 14th intron of OsROS1 led to alternative splicing that generated an extra transcript, mOsROS1, with a 21-nt insertion from the intron. Genetic analyses showed that the tat-1 phenotype is inherited with an unusual gametophytic maternal effect, which is caused not by imprinted gene expression but rather by the presence of the mOsROS1 transcript. Five additional tat alleles with the increased aleurone cell layer and different inheritance patterns were identified by TILLING. Genome-wide bisulfite sequencing revealed general increases in CG and CHG methylations in tat-1 endosperms, along with hypermethylation and reduced expression in two putative aleurone differentiation-related transcription factors. This study thus suggests that OsROS1-mediated DNA demethylation restricts the number of aleurone cell layers in rice and provides a way to improve the nutrition of rice. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | WASHINGTON |
| WOS关键词 | MAIZE ENDOSPERM ; CELL-LAYERS ; GENE ; ARABIDOPSIS ; DIFFERENTIATION ; ENCODES ; PROTEIN ; FORTIFICATION ; CRINKLY4 ; DEMETER |
| 语种 | 英语 |
| 出版者 | NATL ACAD SCIENCES |
| WOS记录号 | WOS:000448713200065 |
| 资助机构 | Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD0100501/2014CB943401] ; Chinese Academy of Sciences (CAS) Innovation Project Molecular Modules for Breeding Design [XDA08010301] ; CAS-Commonwealth Scientific and Industrial Research Organization Bilateral Collaboration Project [GJHZ1406] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20713]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Wu, Xiaoba 2.[Liu, Jinxin 3.Yao, Xuefeng 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.[Yu, Ronald 6.Larkin, Philip J.] CSIRO, Agr & Food, Canberra, ACT 2601, Australia 7.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jinxin,Wu, Xiaoba,Yao, Xuefeng,et al. Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(44):11327-11332. |
| APA | Liu, Jinxin,Wu, Xiaoba,Yao, Xuefeng,Yu, Ronald,Larkin, Philip J.,&Liu, Chun-Ming.(2018).Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(44),11327-11332. |
| MLA | Liu, Jinxin,et al."Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.44(2018):11327-11332. |
入库方式： OAI收割
来源：植物研究所
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