Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass
文献类型：期刊论文
| 作者 | Yu, Juanjuan6; Zhang, Yongxue6; Liu, Junming; Wang, Lin; Liu, Panpan; Yin, Zepeng; Guo, Siyi5; Ma, Jun4; Lu, Zhuang3; Wang, Tai3
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| 刊名 | PLANTA
 |
| 出版日期 | 2018 |
| 卷号 | 248期号:5页码:1079-1099 |
| 关键词 | Germin-like protein Hydrogen peroxide response Proteomics Puccinellia tenuiflora Roots Signaling |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-018-2940-8 |
| 文献子类 | Article |
| 英文摘要 | Main conclusion Hydrogen peroxide-responsive pathways in roots of alkaligrass analyzed by proteomic studies and PutGLP enhance the plant tolerance to saline-, alkali- and cadmium-induced oxidative stresses. Oxidative stress adaptation is critical for plants in response to various stress environments. The halophyte alkaligrass (Puccinellia tenuiflora) is an outstanding pasture with strong tolerance to salt and alkali stresses. In this study, iTRAQ- and 2DE-based proteomics approaches, as well as qRT-PCR and molecular genetics, were employed to investigate H2O2-responsive mechanisms in alkaligrass roots. The evaluation of membrane integrity and reactive oxygen species (ROS)-scavenging systems, as well as abundance patterns of H2O2-responsive proteins/genes indicated that Ca2+-mediated kinase signaling pathways, ROS homeostasis, osmotic modulation, and transcriptional regulation were pivotal for oxidative adaptation in alkaligrass roots. Overexpressing a P. tenuiflora germin-like protein (PutGLP) gene in Arabidopsis seedlings revealed that the apoplastic PutGLP with activities of oxalate oxidase and superoxide dismutase was predominantly expressed in roots and played important roles in ROS scavenging in response to salinity-, alkali-, and CdCl2-induced oxidative stresses. The results provide insights into the fine-tuned redox-responsive networks in halophyte roots. |
| 学科主题 | Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2048 |
| WOS关键词 | HYDROGEN-PEROXIDE STRESS ; PUCCINELLIA-TENUIFLORA ; OXIDATIVE STRESS ; PLASMA-MEMBRANE ; SALT STRESS ; ARABIDOPSIS-THALIANA ; EXPRESSION ANALYSIS ; ANALYSIS REVEALS ; NITRIC-OXIDE ; CROSS-TALK |
| 语种 | 英语 |
| WOS记录号 | WOS:000447030900004 |
| 出版者 | SPRINGER |
| 资助机构 | National Key Research and Development Program [2017YFD0600101] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2572017ET01, 2572017EA05, 2572016AA16] ; Foundation of Shanghai Science and Technology Committee, China [17391900600] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20723]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Plant Mol & Cellular Program, Genet Inst,Dept Biol, Gainesville, FL 32610 USA 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China 4.Henan Univ, Dept Biol, State Key Lab Cotton Biol, Inst Plant Stress Biol, Kaifeng 455000, Peoples R China 5.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 6.Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat Minis, Alkali Soil Nat Environm Sci Ctr, Harbin 150040, Heilongjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Juanjuan,Zhang, Yongxue,Liu, Junming,et al. Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass[J]. PLANTA,2018,248(5):1079-1099. |
| APA | Yu, Juanjuan.,Zhang, Yongxue.,Liu, Junming.,Wang, Lin.,Liu, Panpan.,...&Dai, Shaojun.(2018).Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass.PLANTA,248(5),1079-1099. |
| MLA | Yu, Juanjuan,et al."Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass".PLANTA 248.5(2018):1079-1099. |
入库方式： OAI收割
来源：植物研究所
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