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Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species

文献类型：期刊论文

作者Xue, Zheyong; Tan, Zhengwei3,4; Huang, Ancheng; Zhou, Yuan3; Sun, Juncong3; Wang, Xiaoning1; Thimmappa, Ramesha B.; Stephenson, Michael J.; Osbourn, Anne2; Qi, Xiaoquan
刊名NEW PHYTOLOGIST
出版日期2018
卷号218期号:3页码:1076-1088
关键词chemical diversity conformation orysatinol oxidosqualene cyclase (OSC) parkeol product specificity rice triterpene
ISSN号0028-646X
DOI10.1111/nph.15080
文献子类Article
英文摘要Triterpene synthases, also known as 2,3-oxidosqualene cyclases (OSCs), synthesize diverse triterpene skeletons that form the basis of an array of functionally divergent steroids and triterpenoids. Tetracyclic and pentacyclic triterpene skeletons are synthesized via protosteryl and dammarenyl cations, respectively. The mechanism of conversion between two scaffolds is not well understood. Here, we report a promiscuous OSC from rice (Oryza sativa) (OsOS) that synthesizes a novel pentacyclic triterpene orysatinol as its main product. The OsOS gene is widely distributed in indica subspecies of cultivated rice and in wild rice accessions. Previously, we have characterized a different OSC, OsPS, a tetracyclic parkeol synthase found in japonica subspecies. Phylogenetic and protein structural analyses identified three key amino acid residues (#732, #365, #124) amongst 46 polymorphic sites that determine functional conversion between OsPS and OsOS, specifically, the chair-semi(chair)-chair and chair-boat-chair interconversions. The different orientation of a fourth amino acid residue Y257 was shown to be important for functional conversion The discovery of orysatinol unlocks a new path to triterpene diversity in nature. Our findings also reveal mechanistic insights into the cyclization of oxidosqualene into tetra- and pentacyclic skeletons, and provide a new strategy to identify key residues determining OSC specificity.
学科主题Plant Sciences
出版地HOBOKEN
电子版国际标准刊号1469-8137
WOS关键词OXIDOSQUALENE-LANOSTEROL CYCLASE ; CYCLOARTENOL SYNTHASE ; INFLUENCES CYCLIZATION ; STERIC BULK ; RICE ; BIOSYNTHESIS ; EVOLUTION ; MUTATION ; REARRANGEMENT ; ISOLEUCINE
语种英语
WOS记录号WOS:000430127000022
出版者WILEY
资助机构National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31370337, 31530050] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2013CB127000] ; China Scholarship Council (CSC)China Scholarship Council ; Centre of Excellence for Plant and Microbial Sciences (CEPAMS) ; John Innes Centre ; Chinese Academy of SciencesChinese Academy of Sciences ; UK Biotechnology and Biological Sciences Research Council (BBSRC)UK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) ; Chinese Academy of Sciences (CAS)Chinese Academy of Sciences [CPM11] ; European UnionEuropean Commission [KBBE-2013-7 (TriForC)] ; Joint Engineering and Physical Sciences Research Council/BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC)Engineering & Physical Sciences Research Council (EPSRC) [BB/L014130/1] ; BBSRC Institute Strategic Programme Grant 'Molecules from Nature'UK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/P012523/1] ; John Innes Foundation ; BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/L014130/1, BBS/E/J/000PR9790] Funding Source: UKRI
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/20725]  
专题中科院植物分子生理学重点实验室
作者单位1.Henan Acad Agr Sci, Henan Sesame Res Ctr, Zhengzhou 450002, Henan, Peoples R China
2.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China
3.John Innes Ctr, Dept Metab Biol, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Shandong Univ, Sch Pharmaceut Sci, Minist Educ, Dept Nat Prod Chem,Key Lab Chem Biol, 44 West Wenhua Rd, Jinan 250012, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Xue, Zheyong,Tan, Zhengwei,Huang, Ancheng,et al. Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species[J]. NEW PHYTOLOGIST,2018,218(3):1076-1088.
APA Xue, Zheyong.,Tan, Zhengwei.,Huang, Ancheng.,Zhou, Yuan.,Sun, Juncong.,...&Qi, Xiaoquan.(2018).Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species.NEW PHYTOLOGIST,218(3),1076-1088.
MLA Xue, Zheyong,et al."Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species".NEW PHYTOLOGIST 218.3(2018):1076-1088.

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来源：植物研究所

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