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Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics

文献类型：期刊论文

作者Suo, Jinwei3,5; Zhang, Heng; Zhao, Qi3; Zhang, Nan; Zhang, Yongxue3; Li, Ying3; Song, Baohua3; Yu, Juanjuan3; Cao, Jianguo; Wang, Tai6
刊名GENOMICS PROTEOMICS & BIOINFORMATICS
出版日期2020
卷号18期号:3页码:271-288
关键词Chloroplasts Na2CO3 stress ROS scavenging Phosphoproteomics Puccinellia tenuiflora
ISSN号1672-0229
DOI10.1016/j.gpb.2018.10.011
文献子类Article
英文摘要Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuifiora) under Na2CO3 stress were conducted. In addition, Western blot, real-time PCR, and transgenic techniques were applied to validate the proteomic results and test the functions of the Na2CO3-responsive proteins. A total of 104 and 102 Na2CO3-responsive proteins were identified in leaves and chloroplasts, respectively. In addition, 84 Na2CO3-responsive phosphoproteins were identified, including 56 new phosphorylation sites in 56 phosphoproteins from chloroplasts, which are crucial for the regulation of photosynthesis, ion transport, signal transduction, and energy homeostasis. A full-length PtFBA encoding an alkaligrass chloroplastic fructose-bisphosphate aldolase (FBA) was overexpressed in wild-type cells of cyanobacterium Synechocystis sp. Strain PCC 6803, leading to enhanced Na2CO3 tolerance. All these results indicate that thermal dissipation, state transition, cyclic electron transport, photorespiration, repair of photosystem (PS) II, PSI activity, and ROS homeostasis were altered in response to Na2CO3 stress, which help to improve our understanding of the Na2CO3-responsive mechanisms in halophytes.
学科主题Genetics & Heredity
出版地AMSTERDAM
电子版国际标准刊号2210-3244
WOS关键词COMPARATIVE PROTEOMIC ANALYSIS ; PHOTOSYSTEM-II ; SALT-STRESS ; QUANTITATIVE PROTEOMICS ; NADPH DEHYDROGENASE ; SALINITY TOLERANCE ; ION BALANCE ; PROTEIN ; PHOSPHORYLATION ; LIGHT
WOS研究方向Genetics & Heredity
语种英语
WOS记录号WOS:000605184400005
出版者ELSEVIER
资助机构Foundation of Shanghai Science and Technology CommitteeShanghai Science & Technology Committee [17391900600] ; Program for Professor of Special Appointment (Eastern Scholar) from The Shanghai Bureau of Higher Education (2011) ; Natural and Science Foundation of Heilongjiang Provence [ZD2019C003] ; Shanghai Engineering Research Center of Plant Germplasm Resources [17DZ2252700] ; Program for Professor of Special Appointment (Eastern Scholar) from The Shanghai Bureau of Higher Education (2017)
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/21481]  
专题中科院植物分子生理学重点实验室
作者单位1.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Genet Inst, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL 32610 USA
2.Henan Univ, Inst Plant Stress Biol, Dept Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China
3.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China
4.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China
5.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Minist Educ, Harbin 150040, Peoples R China
6.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China
7.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
8.AB Sciex Asia Pacific Applicat Support Ctr, Shanghai 200233, Peoples R China
9.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China
推荐引用方式
GB/T 7714		 Suo, Jinwei,Zhang, Heng,Zhao, Qi,et al. Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics[J]. GENOMICS PROTEOMICS & BIOINFORMATICS,2020,18(3):271-288.
APA Suo, Jinwei.,Zhang, Heng.,Zhao, Qi.,Zhang, Nan.,Zhang, Yongxue.,...&Dai, Shaojun.(2020).Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics.GENOMICS PROTEOMICS & BIOINFORMATICS,18(3),271-288.
MLA Suo, Jinwei,et al."Na2CO3-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics".GENOMICS PROTEOMICS & BIOINFORMATICS 18.3(2020):271-288.

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来源：植物研究所

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