Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection
文献类型：期刊论文
| 作者 | Ding, Yijuan5; Mei, Jiaqin5; Chai, Yaru4; Yang, Wenjing5; Mao, Yi5; Yan, Baoqin5; Yu, Yang3; Disi, Joseph Onwusemu2; Rana, Kusum5; Li, Jiana5 |
| 刊名 | PLOS PATHOGENS
 |
| 出版日期 | 2020 |
| 卷号 | 16期号:10 |
| ISSN号 | 1553-7366 |
| DOI | 10.1371/journal.ppat.1008919 |
| 文献子类 | Article |
| 英文摘要 | Necrotrophic plant pathogen induces host reactive oxygen species (ROS) production, which leads to necrosis in the host, allowing the pathogen to absorb nutrients from the dead tissues.Sclerotinia sclerotiorumis a typical necrotrophic pathogen that causes Sclerotinia stem rot in more than 400 species, resulting in serious economic losses. Here, we found that threeS.sclerotiorumgenes involved in copper ion import/transport,SsCTR1,SsCCSandSsATX1, were significantly up-regulated during infection ofBrassica oleracea. Function analysis revealed that these genes involved in fungal ROS detoxification and virulence. On the host side, four genes putatively involved in copper ion homeostasis,BolCCS,BolCCH,BolMT2AandBolDRT112, were significantly down-regulated in susceptibleB.oleracea, but stably expressed in resistantB.oleraceaduring infection. Their homologs were found to promote resistance toS.sclerotiorumand increase antioxidant activity inArabidopsis thaliana. Furthermore, copper concentration analysis indicated that copper flow from healthy area into the necrotic area during infection. A model was proposed thatS.sclerotiorumutilizes host copper to detoxify ROS in its cells, whereas the resistant hosts may restrict the supply of essential copper nutrients toS.sclerotiorumby maintaining copper ion homeostasis during infection. Author summary Sclerotinia sclerotiorumis a devastating necrotrophic fungal pathogen and has a substantial economic impact on crop production worldwide.S.sclerotiorumsuccessfully infect the host plants by promote ROS production in host and induce plant necrosis. This raises an interesting question of how this pathogen survive from high level of host-derived ROS. Copper is an essential nutrient for microbial pathogens and serves as an important cofactor for enzymes of scavenging ROS. In our previous study, we simultaneously analyzed the transcriptome changes ofBrassica oleraceaandS.sclerotiorumduring inoculation. The results in present study show thatS.sclerotioruminduces the genes involved in 'copper ion import' and 'copper ion transport' during infection to promote the ROS detoxification and fungal virulence. While, the resistance host maintains the genes involved in 'copper ion homeostasis', which promote the resistance toS.sclerotiorumand antioxidant activity. Furthermore, copper concentration is higher in the necrotic area than the margin and healthy area, which may indicate that copper flow from healthy area into the necrotic area during infection. In summary, our results highlight a more subtle and complex role of copper during the interaction ofS.sclerotiorumand its host plants. |
| 学科主题 | Microbiology ; Parasitology ; Virology |
| 出版地 | SAN FRANCISCO |
| 电子版国际标准刊号 | 1553-7374 |
| WOS关键词 | DIFFERENTIAL EXPRESSION ANALYSIS ; CYTOCHROME-C-OXIDASE ; BRASSICA-NAPUS L. ; SUPEROXIDE-DISMUTASE ; NUTRITIONAL IMMUNITY ; ATX1 GENE ; TOLERANCE ; HOMEOSTASIS ; VIRULENCE ; CHAPERONE |
| WOS研究方向 | Microbiology ; Parasitology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000577091200004 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31671726, 31801395, 31971978] ; Natural Science Foundation of ChongqingNatural Science Foundation of Chongqing [cstc2017shmsxdny80050, cstc2019jcyj-zdxmX0012, cstc2019jcyj-msxmX0486] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [XDJK2018AA004, XDJK2018B022] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21558]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Georgia, Dept Entomol, Athens, GA 30602 USA 2.Southwest Univ, Coll Plant Protect, Chongqing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 4.Southwest Univ, Acad Agr Sci, Chongqing, Peoples R China 5.Southwest Univ, Coll Agron & Biotechnol, Chongqing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Yijuan,Mei, Jiaqin,Chai, Yaru,et al. Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection[J]. PLOS PATHOGENS,2020,16(10). |
| APA | Ding, Yijuan.,Mei, Jiaqin.,Chai, Yaru.,Yang, Wenjing.,Mao, Yi.,...&Qian, Wei.(2020).Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection.PLOS PATHOGENS,16(10). |
| MLA | Ding, Yijuan,et al."Sclerotinia sclerotiorumutilizes host-derived copper for ROS detoxification and infection".PLOS PATHOGENS 16.10(2020). |
入库方式： OAI收割
来源：植物研究所
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