Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner
文献类型：期刊论文
| 作者 | Li, Jinjin1; Yuan, Jinhong1; Wang, Hui; Zhang, Hui3; Zhang, Haiyan |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2020 |
| 卷号 | 71期号:19页码:6174-6186 |
| 关键词 | Arabidopsis copper transporter degradation proteasome ubiquitination vesicle trafficking |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/eraa352 |
| 文献子类 | Article |
| 英文摘要 | The essential nutrient copper is toxic in excess. Therefore, plants must tightly control copper uptake and distribution. Arabidopsis thaliana high-affinity copper transporters (COPTs) mediate copper uptake, partitioning, and redistribution. Here we show that COPT1 localizes to the plasma membrane and endoplasmic reticulum in stably transgenic plants expressing a COPT1-green fluorescent protein (GFP) fusion protein, and the fusion protein is rapidly degraded upon plant exposure to excess copper. MG132 treatment largely abolished copper-induced degradation of COPT1, implying a link between the proteasome and COPT1 activity in modulating copper uptake. Co-immunoprecipitation analyses revealed that COPT1 cannot be ubiquitinated in the presence of excess copper and MG132. Through site-directed mutagenesis, we identified Lys159 in the C-terminal cytoplasmic tail of COPT1 as critical for copper acquisition, but not for copper-mediated down-regulation of COPT1, in plants. Furthermore, pharmacological analysis showed that treatment with a vesicle trafficking inhibitor or a V-ATPase inhibitor does not alter the subcellular dynamics of COPT1-GFP, consistent with the absence of a connection between the endosomal recycling/vacuolar system and COPT1 degradation. Together, our data suggest that proteasomal degradation rather than vacuolar proteolysis is important for the regulation of copper transport to maintain copper homeostasis in plants. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | PLASMA-MEMBRANE ; AUXIN TRANSPORT ; PROTEIN ; ENDOCYTOSIS ; TRAFFICKING ; HOMEOSTASIS ; GROWTH ; IDENTIFICATION ; ABSENCE ; CELLS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000577075400038 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470292] ; Natural Science Foundation of TianjinNatural Science Foundation of Tianjin [18JCZDJC97900] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21559]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jinjin,Yuan, Jinhong,Wang, Hui,et al. Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner[J]. JOURNAL OF EXPERIMENTAL BOTANY,2020,71(19):6174-6186. |
| APA | Li, Jinjin,Yuan, Jinhong,Wang, Hui,Zhang, Hui,&Zhang, Haiyan.(2020).Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner.JOURNAL OF EXPERIMENTAL BOTANY,71(19),6174-6186. |
| MLA | Li, Jinjin,et al."Arabidopsis COPPER TRANSPORTER 1 undergoes degradation in a proteasome-dependent manner".JOURNAL OF EXPERIMENTAL BOTANY 71.19(2020):6174-6186. |
入库方式： OAI收割
来源：植物研究所
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