A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing
文献类型：期刊论文
| 作者 | Ge, Qiang1,4; Tang, Yongyan; Luo, Wei; Zhang, Jingyu; Chong, Kang3; Xu, Yunyuan3 |
| 刊名 | RICE
 |
| 出版日期 | 2020 |
| 卷号 | 13期号:1 |
| 关键词 | OsCYP20-2 Grain length OsSYF2 Spliceosome Alternative splicing |
| ISSN号 | 1939-8425 |
| DOI | 10.1186/s12284-020-00425-0 |
| 文献子类 | Article |
| 英文摘要 | Background Grain size is one of the key agronomic traits that impact grain yield. Several regulatory pathways had been reported to participate in grain size determination via cell expansion or proliferation in rice. However, little is known about cyclophilin and spliceosome participation in grain shape regulation. Results Here, we identified OsCYP20-2, a cyclophilin that influences spliceosome assembly to determine grain length.oscyp20-2 t1, a knock out mutant ofOsCYP20-2caused by T-DNA insertion, produced shorter grains with deficient cell elongation. Through yeast two-hybrid screening and pull-down assays, OsSYF2, a pre-mRNA splicing factor, was identified as an interacting protein of OsCYP20-2. The phenotypes of transgenic lines indicated thatOsSYF2positively regulates grain length via its influence on cell expansion. Transcriptomic analysis showed thatOsSYF2controls the expression and pre-mRNA alternative splicing of genes involved in sugar metabolism. In addition, these two genes have similar effects on panicle architecture. Conclusions Taken together, OsSYF2, an interacting protein of OsCYP20-2, controls grain length and panicle architecture by regulating the alternative splicing of pre-mRNA involved in cell elongation and sugar metabolism. |
| 学科主题 | Agronomy |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1939-8433 |
| WOS关键词 | NATURAL VARIATION ; GENE-EXPRESSION ; RICE ; PROTEIN ; ENCODES ; ARABIDOPSIS ; SIZE ; IDENTIFICATION ; WEIGHT ; YIELD |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| WOS记录号 | WOS:000571813300001 |
| 出版者 | SPRINGER |
| 资助机构 | National Key Research and Development Program [2016YFD0100901] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21579]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China 3.Henan Agr Univ, Coll Agron, Zhengzhou 450046, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Qiang,Tang, Yongyan,Luo, Wei,et al. A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing[J]. RICE,2020,13(1). |
| APA | Ge, Qiang,Tang, Yongyan,Luo, Wei,Zhang, Jingyu,Chong, Kang,&Xu, Yunyuan.(2020).A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing.RICE,13(1). |
| MLA | Ge, Qiang,et al."A Cyclophilin OsCYP20-2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing".RICE 13.1(2020). |
入库方式： OAI收割
来源：植物研究所
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