The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici)
文献类型：期刊论文
| 作者 | Andrzejczak, O. A.; Sorensen, C. K.; Wang, W-Q; Kovalchuk, S.; Hagensen, C. E.; Jensen, O. N.; Carciofi, M.; Hovmoller, M. S.; Rogowska-Wrzesinska, A.; Moller, I. M. |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2020 |
| 卷号 | 10期号:1 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-020-65907-z |
| 文献子类 | Article |
| 英文摘要 | Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding of the response of barley plants to infection by powdery mildew, Blumeria graminis f. sp. tritici, we used quantitative proteomic analysis (LC-MS/MS). We compared the response of two genotypes of barley cultivar Golden Promise, wild type (WT) and plants with overexpression of phytoglobin (previously hemoglobin) class 1 (HO), which has previously been shown to significantly weaken nonhost resistance. A total of 8804 proteins were identified and quantified, out of which the abundance of 1044 proteins changed significantly in at least one of the four comparisons ('i' stands for 'inoculated')- HO/WT and HOi/WTi (giving genotype differences), and WTi/WT and HOi/HO (giving treatment differences). Among these differentially abundant proteins (DAP) were proteins related to structural organization, disease/defense, metabolism, transporters, signal transduction and protein synthesis. We demonstrate that quantitative changes in the proteome can explain physiological changes observed during the infection process such as progression of the mildew infection in HO plants that was correlated with changes in proteins taking part in papillae formation and preinvasion resistance. Overexpression of phytoglobins led to modification in signal transduction prominently by dramatically reducing the number of kinases induced, but also in the turnover of other signaling molecules such as phytohormones, polyamines and Ca2+. Thus, quantitative proteomics broaden our understanding of the role NO and phytoglobins play in barley during nonhost resistance against powdery mildew. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | BERLIN |
| WOS关键词 | NITRIC-OXIDE ; HYPERSENSITIVE RESPONSE ; ARABIDOPSIS-THALIANA ; DISEASE RESISTANCE ; HYDROGEN-PEROXIDE ; PLASMA-MEMBRANE ; HEMOGLOBIN ; EXPRESSION ; HOST ; DEFENSE |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000560458900004 |
| 出版者 | NATURE RESEARCH |
| 资助机构 | Danish Council for Independent Research/ Technology and Production SciencesDet Frie Forskningsrad (DFF) [4005-00073B] ; Novo Nordisk FoundationNovo Nordisk FoundationNovocure Limited [NNF18OC0052451] ; [7292] ; [5072-00007B] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21625]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Carciofi, M. 2.Hovmoller, M. S. 3.Hebelstrup, K. H.] Aarhus Univ, Dept Agroecol, Forsogsvej 1, DK-4200 Slagelse, Denmark 4.[Wang, W-Q 5.Kovalchuk, S. 6.Hagensen, C. E. 7.Jensen, O. N. 8.Rogowska-Wrzesinska, A.] Univ Southern Denmark, Dept Biochem & Mol Biol, Campusvej 55, DK-5230 Odense M, Denmark 9.Rogowska-Wrzesinska, A.] Univ Southern Denmark, VILLUM Ctr Bioanalyt Sci, Campusvej 55, DK-5230 Odense M, Denmark 10.[Carciofi, M. |
| 推荐引用方式 GB/T 7714 | Andrzejczak, O. A.,Sorensen, C. K.,Wang, W-Q,et al. The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici)[J]. SCIENTIFIC REPORTS,2020,10(1). |
| APA | Andrzejczak, O. A..,Sorensen, C. K..,Wang, W-Q.,Kovalchuk, S..,Hagensen, C. E..,...&Hebelstrup, K. H..(2020).The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici).SCIENTIFIC REPORTS,10(1). |
| MLA | Andrzejczak, O. A.,et al."The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici)".SCIENTIFIC REPORTS 10.1(2020). |
入库方式： OAI收割
来源：植物研究所
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