中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 植物研究所
  3. 中国科学院植物研究所
  4. 中科院植物分子生理学重点实验室
Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E

文献类型：期刊论文

作者Zhao, Lu3,4; Li, Wenzheng3,4; Wang, Bingwu3,4; Gao, Yulong3,4; Sui, Xueyi3,4; Liu, Yong3,4; Chen, Xuejun3,4; Yao, Xuefeng1,5; Jiao, Fangchan3,4; Song, Zhongbang3,4
刊名AGRONOMY-BASEL
出版日期2020
卷号10期号:1
关键词eIF4E1-S potyvirus strains Nicotiana tabacum cv. Yunyan87 EMS-induced nonsense mutation TILLING dCAPS markers
DOI10.3390/agronomy10010036
文献子类Article
英文摘要Recessive resistance against potyviruses, such as Potato virus Y (PVY), relies on mutations in the eukaryotic translation initiation factor 4E (eIF4E) or one of its isoforms. The eIF4E1-S mutants of burley tobacco (Nicotiana tabacum L.) exhibit recessive resistance against PVY strains. Here, we developed a TILLING population of flue-cured tobacco (N. tabacum cv. Yunyan87) using ethyl methanesulfonate (EMS) to identify eIF4E1-S mutants. M3 plants homozygous for a nonsense mutation in exon 1 of the eIF4E1-S gene demonstrated resistance against PVYMN. These M3 plants were backcrossed to 'Yunyan87', and BC4F3 plants were screened using derived cleaved amplified polymorphic sequence (dCAPS) markers. BC(4)F(3)( )plants showing agronomic traits comparable to the recurrent parent 'Yunyan87' and resistance against PVYO, PVYN, and PVYNTN strains were identified. These genotypes would provide useful germplasm for future tobacco improvement and would aid in basic research on PVY resistance in flue-cured tobacco.
学科主题Agronomy ; Plant Sciences
电子版国际标准刊号2073-4395
出版地BASEL
WOS关键词POTATO-VIRUS-Y ; RECESSIVE RESISTANCE ; ARABIDOPSIS-THALIANA ; POTYVIRUS RESISTANCE ; GLANDULAR TRICHOMES ; INITIATION-FACTORS ; NATURAL VARIATION ; GENE ; SUSCEPTIBILITY ; EIF(ISO)4E
WOS研究方向Agriculture ; Plant Sciences
语种英语
出版者MDPI
WOS记录号WOS:000513232600036
资助机构Yunnan Academy of Tobacco Agricultural Sciences [2012YN02, 2017YN02, 2017YN03]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/21914]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yunnan Acad Tobacco Agr Sci, Tobacco Breeding & Biotechnol Res Ctr, Kunming 650021, Yunnan, Peoples R China
4.Key Lab Tobacco Biotechnol Breeding, Kunming 650021, Yunnan, Peoples R China
5.Natl Tobacco Genet Engn Res Ctr, Kunming 650021, Yunnan, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Lu,Li, Wenzheng,Wang, Bingwu,et al. Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E[J]. AGRONOMY-BASEL,2020,10(1).
APA Zhao, Lu.,Li, Wenzheng.,Wang, Bingwu.,Gao, Yulong.,Sui, Xueyi.,...&Song, Zhongbang.(2020).Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E.AGRONOMY-BASEL,10(1).
MLA Zhao, Lu,et al."Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E".AGRONOMY-BASEL 10.1(2020).

入库方式： OAI收割

来源：植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。