Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes
文献类型：期刊论文
| 作者 | Song, Zhongbang2; Sui, Xueyi2; Li, Meiyun2; Gao, Yulong2; Li, Wenzheng2; Zhao, Lu2; Li, Feng6; Yao, Xuefeng3,4; Liu, Chunming4,5 ; Wang, Bingwu2
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| 刊名 | PLANT SIGNALING & BEHAVIOR
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| 出版日期 | 2020 |
| 卷号 | 15期号:2 |
| 关键词 | Nornicotine NNN Nicotine demethylation dCAPS marker Tobacco |
| ISSN号 | 1559-2316 |
| DOI | 10.1080/15592324.2019.1710053 |
| 文献子类 | Article |
| 英文摘要 | Substantial progress had been made in reducing nornicotine accumulation in burley tobacco, as nornicotine is a precursor of the carcinogen N-nitrosonornicotine (NNN). Three members of the CYP82E2 family encoding nicotine N-demethylase (NND) have been reported to be responsible for the majority of nicotine demethylation that forms nornicotine in burley tobacco. We had obtained a nonsense mutant of each NND member in flue-cured tobacco from an ethyl methanesulfonate (EMS)-mutagenized population. In this study, we developed dCAPS markers for each nonsense mutation. Using marker-assisted selection, NND mutants were crossed with each other to generate a triple mutant GP449. In line with previous reports, the triple knockout caused significantly decreased levels of nornicotine and NNN in flue-cured tobacco. With the decreased nornicotine, the nicotine level was expected to accumulate. However, the nicotine level in GP449 was significantly decreased to 72.80% of wild type. Realtime RT-PCR analysis showed that the nicotine reduction was correlated with inhibited expression of nicotine biosynthetic pathway genes. The triple mutant and dCAPS markers can be utilized to develop new flue-cured tobacco varieties with lower levels of nornicotine and NNN. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | PHILADELPHIA |
| 电子版国际标准刊号 | 1559-2324 |
| WOS关键词 | ONE-CARBON METABOLISM ; METHYLPUTRESCINE OXIDASE ; TABACUM ; NITROSAMINES ; CONVERSION ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000505440600001 |
| 出版者 | TAYLOR & FRANCIS INC |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31560080] ; China Tobacco Company ; Yunnan Tobacco Company [110201801027, JY-04, 2016YN25, 2017YN03, 2018530000241001] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21961]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China 2.Yunnan Acad Tobacco Agr Sci, Kunming 650021, Yunnan, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 4.Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou, Henan, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Natl Ctr Tobacco Gene Engn, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Zhongbang,Sui, Xueyi,Li, Meiyun,et al. Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes[J]. PLANT SIGNALING & BEHAVIOR,2020,15(2). |
| APA | Song, Zhongbang.,Sui, Xueyi.,Li, Meiyun.,Gao, Yulong.,Li, Wenzheng.,...&Wang, Bingwu.(2020).Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes.PLANT SIGNALING & BEHAVIOR,15(2). |
| MLA | Song, Zhongbang,et al."Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes".PLANT SIGNALING & BEHAVIOR 15.2(2020). |
入库方式： OAI收割
来源：植物研究所
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