The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea
文献类型：期刊论文
| 作者 | Lv, Sulian; Jiang, Ping; Tai, Fang; Wang, Duoliya; Feng, Juanjuan; Fan, Pengxiang; Bao, Hexigeduleng; Li, Yinxin
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| 刊名 | PLANTA
 |
| 出版日期 | 2017 |
| 卷号 | 246期号:6页码:1177-1187 |
| 关键词 | Halophyte Salinity Sodium sequestration Transformation system V-PPase |
| ISSN号 | 0032-0935 |
| DOI | 10.5194/bg-14-3947-2017 |
| 文献子类 | Article |
| 英文摘要 | The V-ATPase subunit A participates in vacuolar Na (+) compartmentalization in Salicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed. |
| 学科主题 | Environmental Sciences & Ecology ; Geology |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1432-2048 |
| WOS关键词 | ROOT PLASMA-MEMBRANE ; H+-PPASE GENE ; SALINITY TOLERANCE ; ENDOPLASMIC-RETICULUM ; PLANT-GROWTH ; ARABIDOPSIS ; TONOPLAST ; PYROPHOSPHATASE ; OVEREXPRESSION ; TRANSPORT |
| 语种 | 英语 |
| WOS记录号 | WOS:000410559400001 |
| 出版者 | SPRINGER |
| 资助机构 | National Research and Development Project of Transgenic Crops of China [2016ZX08009003-002] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270421] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21990]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Sulian,Jiang, Ping,Tai, Fang,et al. The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea[J]. PLANTA,2017,246(6):1177-1187. |
| APA | Lv, Sulian.,Jiang, Ping.,Tai, Fang.,Wang, Duoliya.,Feng, Juanjuan.,...&Li, Yinxin.(2017).The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea.PLANTA,246(6),1177-1187. |
| MLA | Lv, Sulian,et al."The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea".PLANTA 246.6(2017):1177-1187. |
入库方式： OAI收割
来源：植物研究所
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