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Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

文献类型：期刊论文

作者Wang, Shuangshuang1,6; Lu, Jianan1,6; Song, Xiu-Fen; Ren, Shi-Chao; You, Chenjiang1,6; Xu, Jie3; Liu, Chun-Ming; Ma, Hong1,6; Chang, Fang1,6
刊名PLANT PHYSIOLOGY
出版日期2017
卷号175期号:3页码:1186-1202
ISSN号0032-0889
DOI10.1016/j.ecolmodel.2017.07.014
文献子类Article
英文摘要The CLAVATA3/ESR-RELATED (CLE) peptide signals are required for cell-cell communication in several plant growth and developmental processes. However, little is known regarding the possible functions of the CLEs in the anther. Here, we show that a T-DNA insertional mutant, and dominant-negative (DN) and overexpression (OX) transgenic plants of the CLE19 gene, exhibited significantly reduced anther size and pollen grain number and abnormal pollen wall formation in Arabidopsis (Arabidopsis thaliana). Interestingly, the DN-CLE19 pollen grains showed a more extensively covered surface, but CLE19-OX pollen exine exhibited clearly missing connections in the network and lacked separation between areas that normally form the lacunae. With a combination of cell biological, genetic, and transcriptomic analyses on cle19, DN-CLE19, and CLE19-OX plants, we demonstrated that CLE19-OX plants produced highly vacuolated and swollen aborted microspores (ams)-like tapetal cells, lacked lipidic tapetosomes and elaioplasts, and had abnormal pollen primexine without obvious accumulation of sporopollenin precursors. Moreover, CLE19 is important for the normal expression of more than 1,000 genes, including the transcription factor gene AMS, 280 AMS-downstream genes, and other genes involved in pollen coat and pollen exine formation, lipid metabolism, pollen germination, and hormone metabolism. In addition, the DN-CLE19(+/+) ams(-/-) plants exhibited the ams anther phenotype and ams(+/-) partially suppressed the DN-CLE19 transgene-induced pollen exine defects. These findings demonstrate that the proper amount of CLE19 signal is essential for the normal expression of AMS and its downstream gene networks in the regulation of anther development and pollen exine formation.
学科主题Environmental Sciences & Ecology
出版地ROCKVILLE
电子版国际标准刊号1532-2548
WOS关键词RECEPTOR-LIKE KINASES ; EARLY ANTHER DEVELOPMENT ; ABORTED-MICROSPORES AMS ; CELL FATE DETERMINATION ; ARABIDOPSIS-THALIANA ; TAPETUM DEVELOPMENT ; GENE ENCODES ; REPRODUCTIVE DEVELOPMENT ; ENDOSPERM DEVELOPMENT ; PROTEIN-KINASE
语种英语
WOS记录号WOS:000411771800035
出版者AMER SOC PLANT BIOLOGISTS
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31130006, 31470282, 31670316, 31370029] ; State Key Laboratory of Genetic Engineering at Fudan University
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/21993]  
专题中科院植物分子生理学重点实验室
作者单位1.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Minist Educ, Key Lab Biodivers Sci & Ecol Engn, Shanghai 200438, Peoples R China
2.Fudan Univ, Inst Biodivers Sci, Inst Plant Biol, Sch Life Sci, Shanghai 200438, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Collaborat Innovat Ctr Genet & Dev, Shanghai 200240, Peoples R China
5.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
6.Fudan Univ, State Key Lab Genet Engn, Shanghai 200438, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Shuangshuang,Lu, Jianan,Song, Xiu-Fen,et al. Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation[J]. PLANT PHYSIOLOGY,2017,175(3):1186-1202.
APA Wang, Shuangshuang.,Lu, Jianan.,Song, Xiu-Fen.,Ren, Shi-Chao.,You, Chenjiang.,...&Chang, Fang.(2017).Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation.PLANT PHYSIOLOGY,175(3),1186-1202.
MLA Wang, Shuangshuang,et al."Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation".PLANT PHYSIOLOGY 175.3(2017):1186-1202.

入库方式： OAI收割

来源：植物研究所

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