SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean
文献类型：期刊论文
| 作者 | Cai, Bin; Kong, Xiangxiong; Zhong, Chao2; Sun, Suli2; Zhou, Xiao Feng3; Jin, Yin Hua; Wang, Youning4; Li, Xia4; Zhu, Zhendong2; Jin, Jing Bo |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 59期号:1页码:2-14 |
| ISSN号 | 1672-9072 |
| DOI | 10.16476/j.pibb.2017.0012 |
| 文献子类 | Article |
| 英文摘要 | SIZ1 is a small ubiquitin-related modifier (SUMO) E3 ligase that mediates post-translational SUMO modification of target proteins and thereby regulates developmental processes and hormonal and environmental stress responses in Arabidopsis. However, the role of SUMO E3 ligases in crop plants is largely unknown. Here, we identified and characterized two Glycine max (soybean) SUMO E3 ligases, GmSIZ1a and GmSIZ1b. Expression of GmSIZ1a and GmSIZ1b was induced in response to salicylic acid (SA), heat, and dehydration treatment, but not in response to cold, abscisic acid (ABA), and NaCl treatment. Although GmSIZ1a was expressed at higher levels than GmSIZ1b, both genes encoded proteins with SUMO E3 ligase activity in vivo. Heterologous expression of GmSIZ1a or GmSIZ1b rescued the mutant phenotype of Arabidopsis siz1-2, including dwarfism, constitutively activated expression of pathogen-related genes, and ABA-sensitive seed germination. Simultaneous downregulation of GmSIZ1a and GmSIZ1b (GmSIZ1a/b) using RNA interference (RNAi)-mediated gene silencing decreased heat shock-induced SUMO conjugation in soybean. Moreover, GmSIZ1RNAi plants exhibited reduced plant height and leaf size. However, unlike Arabidopsis siz1-2 mutant plants, flowering time and SA levels were not significantly altered in GmSIZ1RNAi plants. Taken together, our results indicate that GmSIZ1a and GmSIZ1b mediate SUMO modification and positively regulate vegetative growth in soybean. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | SALT STRESS RESPONSES ; TRANSCRIPTION FACTOR ; FUNCTIONAL-CHARACTERIZATION ; ARABIDOPSIS ; SIZ1 ; UBIQUITIN ; MODIFIER ; SUMOYLATION ; DROUGHT ; TOLERANCE |
| 语种 | 英语 |
| WOS记录号 | WOS:000397482200005 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471363] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2012CB114302] ; National Transgenic Major Program [2009ZX08009-087B, 2009ZX08009-132B] ; Chinese Academy of SciencesChinese Academy of Sciences [XDA08010105] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22077]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 4.China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Bin,Kong, Xiangxiong,Zhong, Chao,et al. SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2017,59(1):2-14. |
| APA | Cai, Bin.,Kong, Xiangxiong.,Zhong, Chao.,Sun, Suli.,Zhou, Xiao Feng.,...&Jin, Jing Bo.(2017).SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,59(1),2-14. |
| MLA | Cai, Bin,et al."SUMO E3 Ligases GmSIZ1a and GmSIZ1b regulate vegetative growth in soybean".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 59.1(2017):2-14. |
入库方式： OAI收割
来源：植物研究所
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