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Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase

文献类型：期刊论文

作者Ma, Dongming3; Li, Gui; Alejos-Gonzalez, Fatima; Zhu, Yue; Xue, Zhen4; Wang, Aimin4; Zhang, Hui4; Li, Xing5; Ye, Hechun4; Wang, Hong1
刊名PLANT JOURNAL
出版日期2017
卷号91期号:3页码:466-479
关键词Artemisia annua artemisinin arteannuinB artemisinicacid isopentenyl pyrophosphate isomerase isoprene
ISSN号0960-7412
DOI10.1002/2016GB005557
文献子类Article
英文摘要We recently characterized a gene-terpene network that is associated with artemisinin biosynthesis in self-pollinated (SP) Artemisia annua, an effective antimalarial plant. We hypothesize that an alteration of gene expression in the network may improve the production of artemisinin and its precursors. In this study, we cloned an isopentenyl pyrophosphate isomerase (IPPI) cDNA, AaIPPI1, from Artemisia annua (Aa). The full-length cDNA encodes a type-I IPPI containing a plastid transit peptide (PTP) at its amino terminus. After the removal of the PTP, the recombinant truncated AaIPPI1 isomerized isopentenyl pyrophosphate (IPP) to dimethyl allyl pyrophosphate (DMAPP) and vice versa. The steady-state equilibrium ratio of IPP/DMAPP in the enzymatic reactions was approximately 1:7. The truncated AaIPPI1 was overexpressed in the cytosol of the SP A.annua variety. The leaves of transgenic plants produced approximately 4% arteannuinB (gg(-1), dry weight, dw) and 0.17-0.25% artemisinin (gg(-1), dw), the levels of which were significantly higher than those in the leaves of wild-type plants. In addition, transgenic plants showed an increase in artemisinic acid production of more than 1% (gg(-1), dw). In contrast, isoprene formation was significantly reduced in transgenic plants. These results provide evidence that overexpression of AaIPPI1 in the cytosol can lead to metabolic alterations of terpenoid biosynthesis, and show that these transgenic plants have the potential to yield high production levels of arteannuinB as a new precursor source for artemisinin.
学科主题Environmental Sciences & Ecology ; Geology ; Meteorology & Atmospheric Sciences
出版地HOBOKEN
电子版国际标准刊号1365-313X
WOS关键词FARNESYL DIPHOSPHATE SYNTHASE ; ISOPRENOID BIOSYNTHESIS ; MOLECULAR-CLONING ; ESCHERICHIA-COLI ; AMORPHA-4,11-DIENE SYNTHASE ; ANTIMALARIAL-DRUG ; QINGHAOSU ARTEMISININ ; MEVALONATE PATHWAY ; PHOSPHATE-PATHWAY ; DRIED LEAVES
语种英语
WOS记录号WOS:000405103200005
出版者WILEY
资助机构North Carolina Biotechnology Center [550031, 2009-MRG-1117] ; CALS Dean's Enrichment Grants Program, College of Agriculture and Life Science, North Carolina State University, Raleigh, USA ; Knowledge Innovation Program of the Chinese Academy of Sciences, Beijing, P.R. China [KSCX2-SW-329]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/22172]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China
2.Tech Univ Carolo Wilhelmina Braunschweig, Inst Pharmaceut Biol, Braunschweig, Germany
3.North Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA
4.Guangzhou Univ Chinese Med, Res Ctr Chinese Herbal Resource Sci & Engn, Guangzhou, Guangdong, Peoples R China
5.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
6.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Dongming,Li, Gui,Alejos-Gonzalez, Fatima,et al. Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase[J]. PLANT JOURNAL,2017,91(3):466-479.
APA Ma, Dongming.,Li, Gui.,Alejos-Gonzalez, Fatima.,Zhu, Yue.,Xue, Zhen.,...&Xie, De-Yu.(2017).Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase.PLANT JOURNAL,91(3),466-479.
MLA Ma, Dongming,et al."Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase".PLANT JOURNAL 91.3(2017):466-479.

入库方式： OAI收割

来源：植物研究所

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