The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis
文献类型：期刊论文
| 作者 | Zhu, Jia-Ying; Li, Yuyao; Cao, Dong-Mei; Yang, Hongjuan4; Oh, Eunkyoo1; Bi, Yang2; Zhu, Shengwei4 ; Wang, Zhi-Yong
|
| 刊名 | MOLECULAR CELL
 |
| 出版日期 | 2017 |
| 卷号 | 66期号:5页码:648+ |
| ISSN号 | 1097-2765 |
| DOI | 10.3389/fpls.2017.01028 |
| 文献子类 | Article |
| 英文摘要 | The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated brassinosteroid (BR) signaling. Here we identify KIB1 as an F-box E3 ubiquitin ligase that promotes the degradation of BIN2 while blocking its substrate access. Loss-of-function mutations of KIB1 and its homologs abolished BR-induced BIN2 degradation and caused severe BR-insensitive phenotypes. KIB1 directly interacted with BIN2 in a BR-dependent manner and promoted BIN2 ubiquitination in vitro. Expression of an F-box-truncated KIB1 caused BIN2 accumulation but dephosphorylation of its substrate BZR1 and activation of BR responses because KIB1 blocked BIN2 binding to BZR1. Our study demonstrates that KIB1 plays an essential role in BR signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation. |
| 学科主题 | Plant Sciences |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1097-4164 |
| WOS关键词 | PLANT-GROWTH REGULATION ; SIGNAL-TRANSDUCTION ; RECEPTOR KINASE ; UBIQUITIN LIGASE ; GENE-EXPRESSION ; IMAGE-ANALYSIS ; BETA-CATENIN ; BRI1 ; THALIANA ; BZR1 |
| 语种 | 英语 |
| WOS记录号 | WOS:000403487300001 |
| 出版者 | CELL PRESS |
| 资助机构 | NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM066258] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM066258] Funding Source: NIH RePORTER |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22208]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Shanxi Acad Agr Sci, Inst Hort, Taiyuan 030031, Peoples R China 2.Chonnam Natl Univ, Dept Bioenergy Sci & Technol, Gwangju 61186, South Korea 3.Stanford Univ, Dept Biol, Stanford, CA 94305 USA 4.Carnegie Inst Sci, Dept Plant Biol, 290 Panama St, Stanford, CA 94305 USA 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Jia-Ying,Li, Yuyao,Cao, Dong-Mei,et al. The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis[J]. MOLECULAR CELL,2017,66(5):648+. |
| APA | Zhu, Jia-Ying.,Li, Yuyao.,Cao, Dong-Mei.,Yang, Hongjuan.,Oh, Eunkyoo.,...&Wang, Zhi-Yong.(2017).The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis.MOLECULAR CELL,66(5),648+. |
| MLA | Zhu, Jia-Ying,et al."The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis".MOLECULAR CELL 66.5(2017):648+. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。