Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway
文献类型：期刊论文
| 作者 | Liu, Lei; Yan, Xiaojing; Kong, Xiangxiong1; Zhao, Yiqiang; Gong, Zhizhong; Jin, Jing Bo1; Guo, Yan |
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 173期号:1页码:655-667 |
| ISSN号 | 0032-0889 |
| DOI | 10.5194/bg-14-3083-2017 |
| 文献子类 | Article |
| 英文摘要 | The expression of genes with aberrant structure is prevented at both the transcriptional and posttranscriptional regulation levels. Aberrant gene silencing at the posttranscriptional level is well studied; however, it is not well understood how aberrant genes are silenced at the transcriptional level. In this study, through genetic screening a transgenic report line that harbors an aberrant gene (35S-LUC, lacking 39-untranslated region [39-UTR]) and lacks luciferase (LUC) activity, we identify that the small ubiquitin-like modifier (SUMO) protease OTS1 gene is required for maintaining the silence of the reporter 35S-LUC and an endogenous mutator-like element MULE-F19G14 at the transcriptional level, which requires DNA-dependent RNA polymerase (Pol) V and DDR complex, but not Pol IV. The increased transcripts in ots1 mutants are terminated by the 39-UTRs of downstream genes. In addition to ots1 mutations, mutations in several known or putative SUMO proteases and two SUMO E3 ligases, SIZ1 and MMS21, have similar effects on this silencing regulation. Taken together, our results reveal that the enzymes involved in the SUMOylation process restrain aberrant gene transcription by using a downstream gene 39-UTR, and this regulation requires a functional Pol V-dependent pathway in Arabidopsis (Arabidopsis thaliana). |
| 学科主题 | Environmental Sciences & Ecology ; Geology |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | E3 LIGASE SIZ1 ; ARABIDOPSIS-THALIANA ; SALICYLIC-ACID ; OVERLY TOLERANT ; MESSENGER-RNA ; POL IV ; METHYLATION ; SUMOYLATION ; UBIQUITIN ; PLANTS |
| 语种 | 英语 |
| WOS记录号 | WOS:000404404800001 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31430012] ; Foundation for Innovative Research Group of the National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31421062] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22209]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lei,Yan, Xiaojing,Kong, Xiangxiong,et al. Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway[J]. PLANT PHYSIOLOGY,2017,173(1):655-667. |
| APA | Liu, Lei.,Yan, Xiaojing.,Kong, Xiangxiong.,Zhao, Yiqiang.,Gong, Zhizhong.,...&Guo, Yan.(2017).Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway.PLANT PHYSIOLOGY,173(1),655-667. |
| MLA | Liu, Lei,et al."Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway".PLANT PHYSIOLOGY 173.1(2017):655-667. |
入库方式： OAI收割
来源：植物研究所
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