Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning
文献类型：期刊论文
| 作者 | Qu, Xiaolu; Zhang, Ruihui; Zhang, Meng3; Diao, Min3; Xue, Yongbiao1,4; Huang, Shanjin3 |
| 刊名 | MOLECULAR PLANT
 |
| 出版日期 | 2017 |
| 卷号 | 10期号:7页码:930-947 |
| 关键词 | pollen tube clear zone apical actin structure actin dynamics vesicle trafficking myosin |
| ISSN号 | 1674-2052 |
| DOI | 10.1038/s41598-017-07517-w |
| 文献子类 | Article |
| 英文摘要 | Polarized tip growth is a fundamental cellular process in many eukaryotes. In this study, we examined the dynamic restructuring of the actin cytoskeleton and its relationship to vesicle transport during pollen tip growth in Arabidopsis. We found that actin filaments originating from the apical membrane form a specialized structure consisting of longitudinally aligned actin bundles at the cortex and inner cytoplasmic filaments with a distinct distribution. Using actin-based pharmacological treatments and genetic mutants in combination with FRAP (fluorescence recovery after photobleaching) technology to visualize the transport of vesicles within the growth domain of pollen tubes, we demonstrated that cortical actin filaments facilitate tip-ward vesicle transport. We also discovered that the inner apical actin filaments prevent backward movement of vesicles, thus ensuring that sufficient vesicles accumulate at the pollen tube tip to support the rapid growth of the pollen tube. The combinatorial effect of cortical and internal apical actin filaments perfectly explains the generation of the inverted V'' cone-shaped vesicle distribution pattern at the pollen tube tip. When pollen tubes turn, apical actin filaments at the facing side undergo depolymerization and repolymerization to reorient the apical actin structure toward the new growth direction. This actin restructuring precedes vesicle accumulation and changes in tube morphology. Thus, our study provides new insights into the functional relationship between actin dynamics and vesicle transport during rapid and directional pollen tube growth. |
| 学科主题 | Science & Technology - Other Topics |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1752-9867 |
| WOS关键词 | GROWING PLANT-CELLS ; TIP GROWTH ; POLARIZED GROWTH ; ENDOMEMBRANE TRAFFICKING ; FLUORESCENCE RECOVERY ; LILIUM-LONGIFLORUM ; BINDING PROTEINS ; ARABIDOPSIS ; CYTOSKELETON ; DYNAMICS |
| 语种 | 英语 |
| WOS记录号 | WOS:000406980800015 |
| 出版者 | CELL PRESS |
| 资助机构 | Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2013CB945100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31671390, 31471266] ; Tsinghua-Peking Joint Center for Life Sciences ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M571028] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22254]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Xiaolu,Zhang, Ruihui,Zhang, Meng,et al. Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning[J]. MOLECULAR PLANT,2017,10(7):930-947. |
| APA | Qu, Xiaolu,Zhang, Ruihui,Zhang, Meng,Diao, Min,Xue, Yongbiao,&Huang, Shanjin.(2017).Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning.MOLECULAR PLANT,10(7),930-947. |
| MLA | Qu, Xiaolu,et al."Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning".MOLECULAR PLANT 10.7(2017):930-947. |
入库方式： OAI收割
来源：植物研究所
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