Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens
文献类型：期刊论文
| 作者 | Jin, Baolong; Cui, Guanghong; Guo, Juan; Tang, Jinfu; Duan, Lixin1; Lin, Huixin; Shen, Ye; Chen, Tong; Zhang, Huabei; Huang, Luqi |
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 174期号:2页码:943-955 |
| ISSN号 | 0032-0889 |
| DOI | 10.1038/s41598-017-08511-y |
| 文献子类 | Article |
| 英文摘要 | Ent-kaurene diterpenoids are the largest group of known Isodon diterpenoids. Among them, oridonin is accumulated in the leaves, and is the most frequently studied compound because of its antitumor and antibacterial activities. We have identified five copalyl diphosphate synthase (CPS) and six kaurene synthase-like (KSL) genes by transcriptome profiling of Isodon rubescens leaves. An in vitro assay assigns ten of them to five different diterpene biosynthesis pathways, except IrCPS3 that has a mutation in the catalytic motif. The Lamiaceae-specific clade genes (IrCPS1 and IrCPS2) synthesize the intermediate copalyl diphosphate (normal-CPP), while IrCPS4 and IrCPS5 synthesize the intermediate ent-copalyl diphosphate (ent-CPP). IrKSL2, IrKSL4, and IrKSL5 react with ent-CPP to produce an ent-isopimaradiene-like compound, ent-atiserene and ent-kaurene, respectively. Correspondingly, the Lamiaceae-specific clade genes IrKSL1 or IrKSL3 combined with normal-CPP led to the formation of miltiradiene. The compound then underwent aromatization and oxidization with a cytochrome P450 forming two related compounds, abietatriene and ferruginol, which were detected in the root bark. IrKSL6 reacts with normal-CPP to produce isopimaradiene. IrKSL3 and IrKSL6 have the gamma beta alpha tridomain structure, as these proteins tend to possess the bidomain structure of IrKSL1, highlighting the evolutionary history of KSL gene domain loss and further elucidating chemical diversity evolution from a macroevolutionary stance in Lamiaceae. |
| 学科主题 | Science & Technology - Other Topics |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | COPALYL DIPHOSPHATE SYNTHASE ; DITERPENE SYNTHASES ; BIOSYNTHESIS ; EVOLUTION ; ACID ; DISCOVERY ; BIOGEOGRAPHY ; MILTIRADIENE ; DIVERGENCE ; RESOLUTION |
| 语种 | 英语 |
| WOS记录号 | WOS:000407443300014 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | Chinese National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [81325023] ; Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources [2060302] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22258]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao Di Herbs, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Baolong,Cui, Guanghong,Guo, Juan,et al. Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens[J]. PLANT PHYSIOLOGY,2017,174(2):943-955. |
| APA | Jin, Baolong.,Cui, Guanghong.,Guo, Juan.,Tang, Jinfu.,Duan, Lixin.,...&Huang, Luqi.(2017).Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens.PLANT PHYSIOLOGY,174(2),943-955. |
| MLA | Jin, Baolong,et al."Functional Diversification of Kaurene Synthase-Like Genes in Isodon rubescens".PLANT PHYSIOLOGY 174.2(2017):943-955. |
入库方式： OAI收割
来源：植物研究所
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