Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress
文献类型：期刊论文
| 作者 | Guo, Yingtian; Wang, Zhi2; Guan, Xuelian; Hu, Zenghui; Zhang, Ze; Zheng, Jian1; Lu, Yizeng3 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2017 |
| 卷号 | 12期号:8 |
| ISSN号 | 1932-6203 |
| DOI | 10.3389/fpls.2017.01148 |
| 文献子类 | Article |
| 英文摘要 | High temperature is an important environmental factor that affects plant growth and crop yield. Potentilla fruticosa L. has a developed root system and characteristics of resistance to several stresses (e.g., high temperature, cold, drought) that are shared by native shrubs in the north and west of China. To investigate thermotolerance mechanisms in P. fruticosa, 3-year-old plants were subjected to a high temperature of 42 degrees C for 1, 2, and 3 days respectively before analysis. Then, we studied changes in cell ultrastructure using electron microscopy and investigated physiological changes in the leaves of P. fruticosa. Additionally, we used isobaric tags for relative and absolute quantification (iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study proteomic changes in P. fruticosa leaves after 3 d of 42 degrees C heat stress. we found that the cell membrane and structure of chloroplasts, especially the thylakoids in P. fruticosa leaves, was destroyed by a high temperature stress, which might affect the photosynthesis in this species. We identified 35 up-regulated and 23 down-regulated proteins after the heat treatment. Gene Ontology (GO) analysis indicated that these 58 differentially abundant proteins were involved mainly in protein synthesis, protein folding and degradation, abiotic stress defense, photosynthesis, RNA process, signal transduction, and other functions. The 58 proteins fell into different categories based on their subcellular localization mainly in the chloroplast envelope, cytoplasm, nucleus, cytosol, chloroplast, mitochondrion and cell membrane. Five proteins were selected for analysis at the mRNA level; this analysis showed that gene transcription levels were not completely consistent with protein abundance. These results provide valuable information for Potentilla thermotolerance breeding. |
| 学科主题 | Plant Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | ENVELOPE MEMBRANE POLYPEPTIDE ; NUCLEOTIDE-EXCHANGE FACTOR ; ARABIDOPSIS-THALIANA ; PEROXIREDOXIN-Q ; CHLOROPHYLL BREAKDOWN ; OXIDATIVE STRESS ; SHOCK PROTEINS ; EXPRESSION ; TOLERANCE ; RESISTANCE |
| 语种 | 英语 |
| WOS记录号 | WOS:000404602800001 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions [CITTCD20154043] ; Beijing Municipality [CITIDHT20150503] ; Beijing Laboratory of Urban and Rural Ecological Environment [PXM2015-014207000014] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22365]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Beijing Agr Univ, Coll Landscape Architecture, Beijing, Peoples R China 3.Beijing Collaborat Innovat Ctr Ecoenvironm Improv, Beijing, Peoples R China 4.Shandong Forest Germplasm Resources Ctr, Jinan, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Yingtian,Wang, Zhi,Guan, Xuelian,et al. Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress[J]. PLOS ONE,2017,12(8). |
| APA | Guo, Yingtian.,Wang, Zhi.,Guan, Xuelian.,Hu, Zenghui.,Zhang, Ze.,...&Lu, Yizeng.(2017).Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress.PLOS ONE,12(8). |
| MLA | Guo, Yingtian,et al."Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress".PLOS ONE 12.8(2017). |
入库方式： OAI收割
来源：植物研究所
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