Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza
文献类型：期刊论文
| 作者 | Li, Bin3; Cui, Guanghong2; Shen, Guoan3; Zhan, Zhilai2; Huang, Luqi2; Chen, Jiachun; Qi, Xiaoquan3
|
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017 |
| 卷号 | 7 |
| ISSN号 | 2045-2322 |
| DOI | 10.12705/663.3 |
| 文献子类 | Article |
| 英文摘要 | CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report the use of CRISPR/ Cas9 to precisely knock out the committed diterpene synthase gene ( SmCPS1) involved in tanshinone biosynthesis in Salvia miltiorrhiza, a traditional Chinese medicinal herb with significant pharmacological activities, such as vasorelaxation, protection against ischemia-reperfusion injury, and antiarrhythmic effects. Three homozygous and eight chimeric mutants were obtained from 26 independent transgenic hairy root lines by Agrobacterium rhizogenes-mediated transformation. The metabolomic analysis based on LC-qTOF-MS and Q-TRAP-LC-MS/MS revealed that tanshinones, especially cryptotanshinone, tanshinone IIA and tanshinone I, are completely missing in homozygous mutants, without influencing other phenolic acid metabolites. By contrast, tanshinones are decreased but still detectable in chimeric mutants, which is similar to a previously-reported an RNAi study of SmCPS1. These results demonstrate that Agrobacterium rhizogenes-mediated transformation using CRISPR/Cas9 is a simple and efficient genome editing tool in S. miltiorrhiza, thus paving the way for large-scale genome editing in S. miltiorrhiza, which is important for pathway elucidation of secondary metabolites, quality improvement, and yield increases for this valuable traditional Chinese medicinal herb. |
| 学科主题 | Plant Sciences ; Evolutionary Biology |
| 出版地 | LONDON |
| WOS关键词 | AGROBACTERIUM-RHIZOGENES ; HAIRY ROOT ; BIOSYNTHESIS ; TANSHINONES ; EXPRESSION ; BACTERIA ; PLASMID ; PATHWAY ; ARCHAEA ; SYSTEMS |
| 语种 | 英语 |
| WOS记录号 | WOS:000405738400003 |
| 出版者 | NATURE PUBLISHING GROUP |
| 资助机构 | Key Project of Chinese National Programs for Fundamental Research and Development [2013CB127000] ; Chinese National Programs for High Technology Research and Development [2012AA10A304] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22367]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Pharm, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan 430030, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Bin,Cui, Guanghong,Shen, Guoan,et al. Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Li, Bin.,Cui, Guanghong.,Shen, Guoan.,Zhan, Zhilai.,Huang, Luqi.,...&Qi, Xiaoquan.(2017).Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza.SCIENTIFIC REPORTS,7. |
| MLA | Li, Bin,et al."Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：植物研究所
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