Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction
文献类型：期刊论文
| 作者 | Magyar, Melinda; Sipka, Gabor; Kovacs, Laszlo; Ughy, Bettina; Zhu, Qingjun; Han, Guangye; Spunda, Vladimir3,4; Lambrev, Petar H.; Shen, Jian-Ren ; Garab, Gyozo3
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018 |
| 卷号 | 8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-21195-2 |
| 文献子类 | Article |
| 英文摘要 | Photosystem II (PSII) catalyses the photoinduced oxygen evolution and, by producing reducing equivalents drives, in concert with PSI, the conversion of carbon dioxide to sugars. Our knowledge about the architecture of the reaction centre (RC) complex and the mechanisms of charge separation and stabilisation is well advanced. However, our understanding of the processes associated with the functioning of RC is incomplete: the photochemical activity of PSII is routinely monitored by chlorophyll-a fluorescence induction but the presently available data are not free of controversy. In this work, we examined the nature of gradual fluorescence rise of PSII elicited by trains of singleturnover saturating flashes (STSFs) in the presence of a PSII inhibitor, permitting only one stable charge separation. We show that a substantial part of the fluorescence rise originates from lightinduced processes that occur after the stabilisation of charge separation, induced by the first STSF; the temperature-dependent relaxation characteristics suggest the involvement of conformational changes in the additional rise. In experiments using double flashes with variable waiting times (Delta tau) between them, we found that no rise could be induced with zero or short Delta tau, the value of which depended on the temperature -revealing a previously unknown rate-limiting step in PSII. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | LONDON |
| WOS关键词 | OXYGENIC PHOTOSYNTHESIS ; CONFORMATIONAL-CHANGE ; PROTEIN FLEXIBILITY ; HARVESTING COMPLEX ; ENERGY-CONVERSION ; ELECTRON-TRANSFER ; REACTION CENTERS ; CHLOROPLASTS ; YIELD ; TEMPERATURE |
| 语种 | 英语 |
| WOS记录号 | WOS:000424634000009 |
| 出版者 | NATURE PUBLISHING GROUP |
| 资助机构 | Strategic Priority Research ProgramChinese Academy of Sciences [XDB17030100] ; Key Research Project for Frontier Science from CAS, China [QYZDY-SSW-SMC003] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA K 112688, KH 124985, TET_15-1-2016-0144, GINOP-2.3.2-15-2016-00058, OTKA PD 121225] ; Moravian-Silesian Region (Project title: Strengthening international cooperation in science, research and education) [01211/2016/RRC] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20391]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Okayama Univ, Res Inst Interdisciplinary Sci, 1-1,Naka 3 Chome, Tsushima, Okayama 7008530, Japan 2.Hungarian Acad Sci, Biol Res Ctr, Inst Plant Biol, Temesvari Korut 62, H-6726 Szeged, Hungary 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 4.Univ Ostrava, Fac Sci, Dept Phys, Chittussiho 10, CZ-71000 Ostrava, Czech Republic 5.Czech Acad Sci, Global Change Res Inst, Belidla 986-4a, Brno 60300, Czech Republic 6.Okayama Univ, Grad Sch Nat Sci & Technol, 1-1,Naka 3 Chome, Tsushima, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Magyar, Melinda,Sipka, Gabor,Kovacs, Laszlo,et al. Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction[J]. SCIENTIFIC REPORTS,2018,8. |
| APA | Magyar, Melinda.,Sipka, Gabor.,Kovacs, Laszlo.,Ughy, Bettina.,Zhu, Qingjun.,...&Garab, Gyozo.(2018).Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction.SCIENTIFIC REPORTS,8. |
| MLA | Magyar, Melinda,et al."Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction".SCIENTIFIC REPORTS 8(2018). |
入库方式： OAI收割
来源：植物研究所
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