A new method for fast extraction and determination of chlorophylls in natural water
文献类型：期刊论文
| 作者 | Qiu, Nianwei; Wang, Xiushun1,4; Zhou, Feng3 |
| 刊名 | ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
 |
| 出版日期 | 2018 |
| 卷号 | 73期号:1-2页码:77-86 |
| 关键词 | acetone chlorophyll determination dimethyl sulfoxide (DMSO) high-speed refrigerated centrifugation (HSRC) water sample |
| ISSN号 | 0939-5075 |
| DOI | 10.1515/znc-2017-0157 |
| 文献子类 | Article |
| 英文摘要 | Algae collection and chlorophyll extraction are two troublesome steps in the traditional methods used for the determination of chlorophyll concentration in natural water. A new method was established in this study for fast collection and extraction of chlorophyll. Based on our results, the optimum centrifugation condition for collecting algae was determined as: 5000 g for 15 min at 4 degrees C, and the optimum dilution ratio of dimethyl sulfoxide: 90% acetone was 1:4. The specific steps were as follows: the algae in water samples were collected by centrifugation at 5000 g at 4 degrees C for 15 min. The precipitated algae were suspended with 2 mL DMSO. Then the sample was transferred to a 15 mL centrifuge tube, and the tube was incubated at 65 degrees C for 1-2 h in the dark until the sample turned white. After cooling, the chlorophyll extract was diluted with 8 mL 90% acetone, before centrifugation at 5000 g for 5 min. The absorbance values of the supernatants at 750, 664, 647 and 630 nm were used for the calculation of chlorophyll concentrations by the trichromatic equations. This new method saved the filter cost, simplified the extraction process, improved the algae acquisition efficiency, and accelerated the chlorophyll extraction rate. |
| 学科主题 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 出版地 | BERLIN |
| 电子版国际标准刊号 | 1865-7125 |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; PHYTOPLANKTON CHLOROPHYLL ; CAROTENOIDS ; ALGAE ; EQUATIONS ; SOLVENTS |
| 语种 | 英语 |
| WOS记录号 | WOS:000426309100009 |
| 出版者 | WALTER DE GRUYTER GMBH |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471884] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2015JL012, ZR2015DL009] ; Postgraduate Education Innovation Project of Shandong Province [SDYY16087] ; China's Post-doctoral Science FundChina Postdoctoral Science Foundation [2016M592158] ; Refined Experimental Project of Qufu Normal University [JP2014006] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20448]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Nanjing Xiaozhuang Univ, Sch Food Sci, Nanjing 211171, Jiangsu, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qufu Normal Univ, Sch Life Sci, Qufu, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Nianwei,Wang, Xiushun,Zhou, Feng. A new method for fast extraction and determination of chlorophylls in natural water[J]. ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES,2018,73(1-2):77-86. |
| APA | Qiu, Nianwei,Wang, Xiushun,&Zhou, Feng.(2018).A new method for fast extraction and determination of chlorophylls in natural water.ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES,73(1-2),77-86. |
| MLA | Qiu, Nianwei,et al."A new method for fast extraction and determination of chlorophylls in natural water".ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES 73.1-2(2018):77-86. |
入库方式： OAI收割
来源：植物研究所
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