Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing
文献类型：期刊论文
| 作者 | Zhang, Yuling; Giuliani, Rita8; Zhang, Youjun1; Zhang, Yang2; Araujo, Wagner Luiz1; Wang, Baichen7; Liu, Peng6; Sun, Qi5; Cousins, Asaph8; Edwards, Gerald8 |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 60期号:8页码:670-690 |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.12656 |
| 文献子类 | Article |
| 英文摘要 | In C-4 photosynthesis, pyruvate orthophosphate dikinase (PPDK) catalyzes the regeneration of phosphoenolpyruvate in the carbon shuttle pathway. Although the biochemical function of PPDK in maize is well characterized, a genetic analysis of PPDK has not been reported. In this study, we use the maize transposable elements Mutator and Ds to generate multiple mutant alleles of PPDK. Loss-of-function mutants are seedling lethal, even when plants were grown under 2% CO2, and they show very low capacity for CO2 assimilation, indicating C-4 photosynthesis is essential in maize. Using RNA-seq and GC-MS technologies, we examined the transcriptional and metabolic responses to a deficiency in PPDK activity. These results indicate loss of PPDK results in downregulation of gene expression of enzymes of the C-4 cycle, the Calvin cycle, and components of photochemistry. Furthermore, the loss of PPDK did not change Kranz anatomy, indicating that this metabolic defect in the C-4 cycle did not impinge on the morphological differentiation of C-4 characters. However, sugar metabolism and nitrogen utilization were altered in the mutants. An interaction between light intensity and genotype was also detected from transcriptome profiling, suggesting altered transcriptional and metabolic responses to environmental and endogenous signals in the PPDK mutants. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | NADP-MALATE DEHYDROGENASE ; BUNDLE-SHEATH-CELLS ; C-4 PHOTOSYNTHESIS ; PYRUVATE,ORTHOPHOSPHATE DIKINASE ; DIFFERENTIAL EXPRESSION ; NITROGEN REMOBILIZATION ; GAS-CHROMATOGRAPHY ; GLOBAL FOOD ; ZEA-MAYS ; ARABIDOPSIS |
| 语种 | 英语 |
| WOS记录号 | WOS:000440682400004 |
| 出版者 | WILEY |
| 资助机构 | NSFCNational Natural Science Foundation of China (NSFC) [31271393] ; National Key Research and Developmen Program of China [2016YFD0101003] ; Taishan Program ; NSFNational Science Foundation (NSF) [EF-1105249, IOS-092270, IOS-1127017] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20474]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Washington State Univ, Sch Biol Sci, Mol Plant Sci, Pullman, WA 99164 USA 2.Max Planck Inst Mol Pflanzenphysiol, Muhlenberg 1, D-14476 Potsdam, Germany 3.Brutnell, Thomas P.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA 4.Cornell Univ, Life Sci Core Labs Ctr, Computat Biol Serv Unit, Ithaca, NY 14850 USA 5.Iowa State Univ, Dept Stat, Ames, IA 50011 USA 6.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 7.Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA 8.Shandong Agr Univ, Coll Agron Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuling,Giuliani, Rita,Zhang, Youjun,et al. Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2018,60(8):670-690. |
| APA | Zhang, Yuling.,Giuliani, Rita.,Zhang, Youjun.,Zhang, Yang.,Araujo, Wagner Luiz.,...&Li, Pinghua.(2018).Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,60(8),670-690. |
| MLA | Zhang, Yuling,et al."Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 60.8(2018):670-690. |
入库方式： OAI收割
来源：植物研究所
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