Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga
文献类型：期刊论文
| 作者 | Pi, Xiong; Tian, Lirong; Dai, Huai-En; Qin, Xiaochun; Cheng, Lingpeng; Kuang, Tingyun; Sui, Sen-Fang; Shen, Jian-Ren
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| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2018 |
| 卷号 | 115期号:17页码:4423-4428 |
| 关键词 | cryo-EM PSI-LHCR red algae PsaO energy transfer |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1722482115 |
| 文献子类 | Article |
| 英文摘要 | Photosystem I (PSI) is one of the two photosystems present in oxygenic photosynthetic organisms and functions to harvest and convert light energy into chemical energy in photosynthesis. In eukaryotic algae and higher plants, PSI consists of a core surrounded by variable species and numbers of light-harvesting complex (LHC)I proteins, forming a PSI-LHCI supercomplex. Here, we report cryo-EM structures of PSI-LHCR from the red alga Cyanidioschyzon merolae in two forms, one with three Lhcr subunits attached to the side, similar to that of higher plants, and the other with two additional Lhcr subunits attached to the opposite side, indicating an ancient form of PSI-LHCI. Furthermore, the red algal PSI core showed features of both cyanobacterial and higher plant PSI, suggesting an intermediate type during evolution from prokaryotes to eukaryotes. The structure of PsaO, existing in eukaryotic organisms, was identified in the PSI core and binds three chlorophylls a and may be important in harvesting energy and in mediating energy transfer from LHCII to the PSI core under state-2 conditions. Individual attaching sites of LHCRs with the core subunits were identified, and each Lhcr was found to contain 11 to 13 chlorophylls a and 5 zeaxanthins, which are apparently different from those of LHCs in plant PSI-LHCI. Together, our results reveal unique energy transfer pathways different from those of higher plant PSI-LHCI, its adaptation to the changing environment, and the possible changes of PSI-LHCI during evolution from prokaryotes to eukaryotes. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | WASHINGTON |
| WOS关键词 | ENERGY-TRANSFER PATHWAYS ; CHLAMYDOMONAS-REINHARDTII ; CYANIDIOSCHYZON-MEROLAE ; ANGSTROM RESOLUTION ; OXYGENIC PHOTOSYNTHESIS ; LHCI SUPERCOMPLEX ; CRYSTAL-STRUCTURE ; CYTOCHROME C(6) ; COMPLEXES ; EVOLUTION |
| 语种 | 英语 |
| WOS记录号 | WOS:000430697500061 |
| 出版者 | NATL ACAD SCIENCES |
| 资助机构 | National Key R&D Program of China [2017YFA0503700] ; National Basic Research Program of ChinaNational Basic Research Program of China [2016YFA0501100, 2015CB150100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31230016, 31370717, 31622007, 31670237] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDB17000000] ; Chinese Academy of Sciences Key Research Project for Frontier Science [QYZDY-SSW-SMC003] ; Japan Society for the Promotion of ScienceMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [JP17H0643419] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20524]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Dai, Huai-En 2.Kuang, Tingyun 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 4.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan 5.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan 6.[Tian, Lirong 7.Qin, Xiaochun 8.[Pi, Xiong 9.Cheng, Lingpeng 10.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pi, Xiong,Tian, Lirong,Dai, Huai-En,et al. Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(17):4423-4428. |
| APA | Pi, Xiong.,Tian, Lirong.,Dai, Huai-En.,Qin, Xiaochun.,Cheng, Lingpeng.,...&Shen, Jian-Ren.(2018).Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(17),4423-4428. |
| MLA | Pi, Xiong,et al."Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.17(2018):4423-4428. |
入库方式： OAI收割
来源：植物研究所
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