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ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings

文献类型：期刊论文

作者Jiang, Tian3; Zhang, Jing3; Rong, Liwei3; Feng, Yanjiang1; Wang, Qi1; Song, Qiulai1; Zhang, Lixin3; Ouyang, Min
刊名JOURNAL OF EXPERIMENTAL BOTANY
出版日期2018
卷号69期号:12页码:3037-3051
关键词Arabidopsis chloroplast development cotyledon Early Chloroplast Development 1 (ECD1) early stages pentatricopeptide repeat protein (PPR) RNA editing
ISSN号0022-0957
DOI10.1093/jxb/ery139
文献子类Article
英文摘要Chloroplast development is a highly complex process and the regulatory mechanisms have not yet been fully characterized. In this study, we identified Early Chloroplast Development 1 (ECD1), a chloroplast-localized pentatricopeptide repeat protein (PPR) belonging to the PLS subfamily. Inactivation of ECD1 in Arabidopsis led to embryo lethality, and abnormal embryogenesis occurred in ecdl/+ heterozygous plants. A decrease in ECD1 expression induced by RNAi resulted in seedlings with albino cotyledons but normal true leaves. The aberrant morphology and under-developed thylakoid membrane system in cotyledons of RNAi seedlings suggests a role of ECD1 specifically in chloroplast development in seedlings. In cotyledons of ECD1-RNAi plants, RNA-editing of rps14-149 (encoding ribosomal protein S14) was seriously impaired. In addition, dramatically decreased plastid-encoded RNA polymerase-dependent gene expression and abnormal chloroplast rRNA processing were also observed. Taken together, our results indicate that ECD1 is indispensable for chloroplast development at the seedling stage in Arabidopsis.
学科主题Plant Sciences
出版地OXFORD
电子版国际标准刊号1460-2431
WOS关键词PENTATRICOPEPTIDE REPEAT PROTEINS ; RIBOSOME-DEFICIENT PLASTIDS ; GENE-EXPRESSION ; ARABIDOPSIS-THALIANA ; PPR PROTEIN ; DIFFERENTIAL REGULATION ; EMBRYO DEVELOPMENT ; PLANT DEVELOPMENT ; ESCHERICHIA-COLI ; MESSENGER-RNA
语种英语
WOS记录号WOS:000434047800015
出版者OXFORD UNIV PRESS
资助机构State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150100] ; Youth Innovation Promotion Association CAS [2013059]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/20573]  
专题中科院光生物学重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Heilongjiang Acad Agr Sci, Cultivat & Crop Tillage Inst, Harbin 150086, Heilongjiang, Peoples R China
3.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Tian,Zhang, Jing,Rong, Liwei,et al. ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(12):3037-3051.
APA Jiang, Tian.,Zhang, Jing.,Rong, Liwei.,Feng, Yanjiang.,Wang, Qi.,...&Ouyang, Min.(2018).ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings.JOURNAL OF EXPERIMENTAL BOTANY,69(12),3037-3051.
MLA Jiang, Tian,et al."ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings".JOURNAL OF EXPERIMENTAL BOTANY 69.12(2018):3037-3051.

入库方式： OAI收割

来源：植物研究所

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