Large-scale Proteomic and Phosphoproteomic Analyses of Maize Seedling Leaves During De-etiolation
文献类型：期刊论文
| 作者 | Gao, Zhi-Fang; Shen, Zhuo4; Chao, Qing; Yan, Zhen; Ge, Xuan-Liang; Lu, Tiancong1; Zheng, Haiyan6; Qian, Chun-Rong; Wang, Bai-Chen |
| 刊名 | GENOMICS PROTEOMICS & BIOINFORMATICS
 |
| 出版日期 | 2020 |
| 卷号 | 18期号:4页码:397-414 |
| 关键词 | Maize seedling leaves De-etiolation Quantitative analysis Proteome Phosphoproteome |
| ISSN号 | 1672-0229 |
| DOI | 10.1016/j.gpb.2020.12.004 |
| 文献子类 | Article |
| 英文摘要 | De-etiolation consists of a series of developmental and physiological changes that a plant undergoes in response to light. During this process light, an important environmental signal, triggers the inhibition of mesocotyl elongation and the production of photosynthetically active chloroplasts, and etiolated leaves transition from the sink stage to the source stage. De-etiolation has been extensively studied in maize (Zea mays L.). However, little is known about how this transition is regulated. In this study, we described a quantitative proteomic and phosphoproteomic atlas of the de-etiolation process in maize. We identified 16,420 proteins in proteome, among which 14,168 proteins were quantified. In addition, 8746 phosphorylation sites within 3110 proteins were identified. From the combined proteomic and phosphoproteomic data, we identified a total of 17,436 proteins. Only 7.0% (998/14,168) of proteins significantly changed in abundance during de-etiolation. In contrast, 26.6% of phosphorylated proteins exhibited significant changes in phosphorylation level; these included proteins involved in gene expression and homeostatic pathways and rate-limiting enzymes involved in photosynthetic light and carbon reactions. Based on phosphoproteomic analysis, 34.0% (1057/3110) of phosphorylated proteins identified in this study contained more than 2 phosphorylation sites, and 37 proteins contained more than 16 phosphorylation sites, indicating that multi-phosphorylation is ubiquitous during the de-etiolation process. Our results suggest that plants might preferentially regulate the level of posttranslational modifications (PTMs) rather than protein abundance for adapting to changing environments. The study of PTMs could thus better reveal the regulation of de-etiolation. |
| 学科主题 | Genetics & Heredity |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 2210-3244 |
| WOS关键词 | POSTTRANSLATIONAL MODIFICATIONS ; PROTEIN-PHOSPHORYLATION ; BLUE-LIGHT ; PLANT PHOSPHOPROTEOMICS ; SIGNAL TRANSDUCER ; ARABIDOPSIS CRY2 ; RICE SEEDLINGS ; BUNDLE-SHEATH ; PHYTOCHROME-A ; ACCLIMATION |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000667272600004 |
| 出版者 | ELSEVIER |
| 资助机构 | National Key R AMP ; D Program of China [2016YFD0101003] ; Heilongjiang Provincial Outstanding Youth Science Foundation, China [JC2017008] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21461]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Farming, Harbin 150086, Peoples R China 2.Rutgers State Univ, Ctr Adv Biotechnol & Med, Biol Mass Spectrometry Facil, Piscataway, NJ 08855 USA 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, CAS, Beijing 100093, Peoples R China 5.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Peoples R China 6.Beijing Prot World Biotech, Beijing 100012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Zhi-Fang,Shen, Zhuo,Chao, Qing,et al. Large-scale Proteomic and Phosphoproteomic Analyses of Maize Seedling Leaves During De-etiolation[J]. GENOMICS PROTEOMICS & BIOINFORMATICS,2020,18(4):397-414. |
| APA | Gao, Zhi-Fang.,Shen, Zhuo.,Chao, Qing.,Yan, Zhen.,Ge, Xuan-Liang.,...&Wang, Bai-Chen.(2020).Large-scale Proteomic and Phosphoproteomic Analyses of Maize Seedling Leaves During De-etiolation.GENOMICS PROTEOMICS & BIOINFORMATICS,18(4),397-414. |
| MLA | Gao, Zhi-Fang,et al."Large-scale Proteomic and Phosphoproteomic Analyses of Maize Seedling Leaves During De-etiolation".GENOMICS PROTEOMICS & BIOINFORMATICS 18.4(2020):397-414. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。